RA of bacteria identified at species level. RA of species per orthopedic material specimen per case and of four negative controls. PJI—periprosthetic joint infection. Total richness represents the sum of OTUs for the patients sequencing analysis. ‘*’ indicates species that were cultured clinically.
| PJI-1 . | Suture . | Femoral . | Tibial . | Patellar . | Articulating surface . | Bone cement spacer . | Tibial screws . |
|---|---|---|---|---|---|---|---|
| . | (Su) . | (FC) . | (TC) . | (P) . | (AS) . | (BCS) . | (TS) . |
| B. fragilis* | 66.82% | 98.75% | 92.24% | 88.42% | 87.54% | ||
| P. aeruginosa | 23.41% | 0.38% | 2.97% | 4.38% | 11.36% | ||
| Steno. maltophilia* | 1.17% | 0.87% | 4.30% | 6.83% | 0.60% | ||
| S. aureus* | 8.12% | 0.00% | 0.01% | 0.01% | 0.02% | ||
| Oxalobacter spp. | 0.48% | 0.00% | 0.48% | 0.36% | 0.47% | ||
| PJI-2 | Suture | Femoral | Tibial | Patellar | Articulating surface | Bone cement spacer | Tibial screws |
| (Su) | (FC) | (TC) | (P) | (AS) | (BCS) | (TS) | |
| P. aeruginosa | 99.15% | 0.21% | |||||
| Strep. agalactiae* | 0.04% | 95.11% | |||||
| S. aureus | 0.78% | 1.50% | |||||
| Prop. acnes | 0.02% | 2.03% | |||||
| S. epidermidis* | 0.00% | 1.14% | |||||
| PJI-3 | Suture | Femoral | Tibial | Patellar | Articulating surface | Bone cement spacer | Tibial screws |
| (Su) | (FC) | (TC) | (P) | (AS) | (BCS) | (TS) | |
| S. aureus* | 96.77% | 26.72% | 63.59% | 85.70% | 99.03% | 97.96% | |
| P. aeruginosa | 2.84% | 73.05% | 36.15% | 13.97% | 0.66% | 1.73% | |
| B. fragilis | 0.39% | 0.23% | 0.27% | 0.33% | 0.31% | 0.31% | |
| Negative control | |||||||
| P. tolaasii | 37.00% | ||||||
| Prop. acnes | 22.61% | ||||||
| V. paradoxus | 8.22% | ||||||
| R. pickettii | 7.15% | ||||||
| Strep. thermophilus | 6.92% | ||||||
| R. insidiosa | 3.64% | ||||||
| Staphylococcus spp. | 3.15% | ||||||
| P. putida | 2.72% | ||||||
| Stenotrophomonas spp. | 2.39% | ||||||
| Achromobacter xylosoxidans | 1.71% | ||||||
| Propionibacterium spp. | 1.15% | ||||||
| Acinetobacter johnsonii | 1.14% | ||||||
| Delftia spp. | 1.11% | ||||||
| Hydrogenophilus spp. | 0.7% | ||||||
| Rubrobacter spp. | 0.4% |
| PJI-1 . | Suture . | Femoral . | Tibial . | Patellar . | Articulating surface . | Bone cement spacer . | Tibial screws . |
|---|---|---|---|---|---|---|---|
| . | (Su) . | (FC) . | (TC) . | (P) . | (AS) . | (BCS) . | (TS) . |
| B. fragilis* | 66.82% | 98.75% | 92.24% | 88.42% | 87.54% | ||
| P. aeruginosa | 23.41% | 0.38% | 2.97% | 4.38% | 11.36% | ||
| Steno. maltophilia* | 1.17% | 0.87% | 4.30% | 6.83% | 0.60% | ||
| S. aureus* | 8.12% | 0.00% | 0.01% | 0.01% | 0.02% | ||
| Oxalobacter spp. | 0.48% | 0.00% | 0.48% | 0.36% | 0.47% | ||
| PJI-2 | Suture | Femoral | Tibial | Patellar | Articulating surface | Bone cement spacer | Tibial screws |
| (Su) | (FC) | (TC) | (P) | (AS) | (BCS) | (TS) | |
| P. aeruginosa | 99.15% | 0.21% | |||||
| Strep. agalactiae* | 0.04% | 95.11% | |||||
| S. aureus | 0.78% | 1.50% | |||||
| Prop. acnes | 0.02% | 2.03% | |||||
| S. epidermidis* | 0.00% | 1.14% | |||||
| PJI-3 | Suture | Femoral | Tibial | Patellar | Articulating surface | Bone cement spacer | Tibial screws |
| (Su) | (FC) | (TC) | (P) | (AS) | (BCS) | (TS) | |
| S. aureus* | 96.77% | 26.72% | 63.59% | 85.70% | 99.03% | 97.96% | |
| P. aeruginosa | 2.84% | 73.05% | 36.15% | 13.97% | 0.66% | 1.73% | |
| B. fragilis | 0.39% | 0.23% | 0.27% | 0.33% | 0.31% | 0.31% | |
| Negative control | |||||||
| P. tolaasii | 37.00% | ||||||
| Prop. acnes | 22.61% | ||||||
| V. paradoxus | 8.22% | ||||||
| R. pickettii | 7.15% | ||||||
| Strep. thermophilus | 6.92% | ||||||
| R. insidiosa | 3.64% | ||||||
| Staphylococcus spp. | 3.15% | ||||||
| P. putida | 2.72% | ||||||
| Stenotrophomonas spp. | 2.39% | ||||||
| Achromobacter xylosoxidans | 1.71% | ||||||
| Propionibacterium spp. | 1.15% | ||||||
| Acinetobacter johnsonii | 1.14% | ||||||
| Delftia spp. | 1.11% | ||||||
| Hydrogenophilus spp. | 0.7% | ||||||
| Rubrobacter spp. | 0.4% |
RA of bacteria identified at species level. RA of species per orthopedic material specimen per case and of four negative controls. PJI—periprosthetic joint infection. Total richness represents the sum of OTUs for the patients sequencing analysis. ‘*’ indicates species that were cultured clinically.
| PJI-1 . | Suture . | Femoral . | Tibial . | Patellar . | Articulating surface . | Bone cement spacer . | Tibial screws . |
|---|---|---|---|---|---|---|---|
| . | (Su) . | (FC) . | (TC) . | (P) . | (AS) . | (BCS) . | (TS) . |
| B. fragilis* | 66.82% | 98.75% | 92.24% | 88.42% | 87.54% | ||
| P. aeruginosa | 23.41% | 0.38% | 2.97% | 4.38% | 11.36% | ||
| Steno. maltophilia* | 1.17% | 0.87% | 4.30% | 6.83% | 0.60% | ||
| S. aureus* | 8.12% | 0.00% | 0.01% | 0.01% | 0.02% | ||
| Oxalobacter spp. | 0.48% | 0.00% | 0.48% | 0.36% | 0.47% | ||
| PJI-2 | Suture | Femoral | Tibial | Patellar | Articulating surface | Bone cement spacer | Tibial screws |
| (Su) | (FC) | (TC) | (P) | (AS) | (BCS) | (TS) | |
| P. aeruginosa | 99.15% | 0.21% | |||||
| Strep. agalactiae* | 0.04% | 95.11% | |||||
| S. aureus | 0.78% | 1.50% | |||||
| Prop. acnes | 0.02% | 2.03% | |||||
| S. epidermidis* | 0.00% | 1.14% | |||||
| PJI-3 | Suture | Femoral | Tibial | Patellar | Articulating surface | Bone cement spacer | Tibial screws |
| (Su) | (FC) | (TC) | (P) | (AS) | (BCS) | (TS) | |
| S. aureus* | 96.77% | 26.72% | 63.59% | 85.70% | 99.03% | 97.96% | |
| P. aeruginosa | 2.84% | 73.05% | 36.15% | 13.97% | 0.66% | 1.73% | |
| B. fragilis | 0.39% | 0.23% | 0.27% | 0.33% | 0.31% | 0.31% | |
| Negative control | |||||||
| P. tolaasii | 37.00% | ||||||
| Prop. acnes | 22.61% | ||||||
| V. paradoxus | 8.22% | ||||||
| R. pickettii | 7.15% | ||||||
| Strep. thermophilus | 6.92% | ||||||
| R. insidiosa | 3.64% | ||||||
| Staphylococcus spp. | 3.15% | ||||||
| P. putida | 2.72% | ||||||
| Stenotrophomonas spp. | 2.39% | ||||||
| Achromobacter xylosoxidans | 1.71% | ||||||
| Propionibacterium spp. | 1.15% | ||||||
| Acinetobacter johnsonii | 1.14% | ||||||
| Delftia spp. | 1.11% | ||||||
| Hydrogenophilus spp. | 0.7% | ||||||
| Rubrobacter spp. | 0.4% |
| PJI-1 . | Suture . | Femoral . | Tibial . | Patellar . | Articulating surface . | Bone cement spacer . | Tibial screws . |
|---|---|---|---|---|---|---|---|
| . | (Su) . | (FC) . | (TC) . | (P) . | (AS) . | (BCS) . | (TS) . |
| B. fragilis* | 66.82% | 98.75% | 92.24% | 88.42% | 87.54% | ||
| P. aeruginosa | 23.41% | 0.38% | 2.97% | 4.38% | 11.36% | ||
| Steno. maltophilia* | 1.17% | 0.87% | 4.30% | 6.83% | 0.60% | ||
| S. aureus* | 8.12% | 0.00% | 0.01% | 0.01% | 0.02% | ||
| Oxalobacter spp. | 0.48% | 0.00% | 0.48% | 0.36% | 0.47% | ||
| PJI-2 | Suture | Femoral | Tibial | Patellar | Articulating surface | Bone cement spacer | Tibial screws |
| (Su) | (FC) | (TC) | (P) | (AS) | (BCS) | (TS) | |
| P. aeruginosa | 99.15% | 0.21% | |||||
| Strep. agalactiae* | 0.04% | 95.11% | |||||
| S. aureus | 0.78% | 1.50% | |||||
| Prop. acnes | 0.02% | 2.03% | |||||
| S. epidermidis* | 0.00% | 1.14% | |||||
| PJI-3 | Suture | Femoral | Tibial | Patellar | Articulating surface | Bone cement spacer | Tibial screws |
| (Su) | (FC) | (TC) | (P) | (AS) | (BCS) | (TS) | |
| S. aureus* | 96.77% | 26.72% | 63.59% | 85.70% | 99.03% | 97.96% | |
| P. aeruginosa | 2.84% | 73.05% | 36.15% | 13.97% | 0.66% | 1.73% | |
| B. fragilis | 0.39% | 0.23% | 0.27% | 0.33% | 0.31% | 0.31% | |
| Negative control | |||||||
| P. tolaasii | 37.00% | ||||||
| Prop. acnes | 22.61% | ||||||
| V. paradoxus | 8.22% | ||||||
| R. pickettii | 7.15% | ||||||
| Strep. thermophilus | 6.92% | ||||||
| R. insidiosa | 3.64% | ||||||
| Staphylococcus spp. | 3.15% | ||||||
| P. putida | 2.72% | ||||||
| Stenotrophomonas spp. | 2.39% | ||||||
| Achromobacter xylosoxidans | 1.71% | ||||||
| Propionibacterium spp. | 1.15% | ||||||
| Acinetobacter johnsonii | 1.14% | ||||||
| Delftia spp. | 1.11% | ||||||
| Hydrogenophilus spp. | 0.7% | ||||||
| Rubrobacter spp. | 0.4% |
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