Nucleotide sequences of the primers used for quantitative reverse transcription-PCR
| Gene1 . | Sequence (5′-3′) . | . | Accession no. . |
|---|---|---|---|
| MyHC I2 | Forward | GGC CCC TTC CAG CTT GA | L10129 |
| Reverse | TGG CTG CGC CTT GGT TT | ||
| MyHC IIa2 | Forward | TTA AAA AGC TCC AAG AAC TGT TTC A | U11772 |
| Reverse | CCA TTT CCT GGT CGG AAC TC | ||
| MyHC IIx2 | Forward | TGC TTC AAG TTC TGC CCC ACT | U90719 |
| Reverse | GGC TGC GGG TTA TTG ATG G | ||
| MyHC IIb2 | Forward | CAC TTT AAG TAG TTG TCT GCC TTG | U90720 |
| Reverse | AG GGC AGC AGG GCA CTA GAT GT | ||
| β-Actin | Forward | GGC ATC CAC GAG ACC ACC TTC AA | U07786 |
| Reverse | CAG ACA GCA CCG TGT TGG CGT AGA | ||
| Citrate synthase | Forward | TGC CAG TGC TTC TTC CAC GAA CTT | NM_214276 |
| Reverse | GTT GCC GTG TTG CTG CCT GAA G | ||
| PPARα | Forward | CAA GGG CTT CTT TCG GAG AAC CAT | AF175309 |
| Reverse | GCG CCC AAA TCG AAT TGC ATT AT | ||
| LDH | Forward | GTG CAT CCG ATT TCC ACC ATG ATT | U07178 |
| Reverse | CCA TTC TGT CCC AAG ATG CAA GGA | ||
| Glycogen synthase | Forward | CCC AGT GGG AGG AGG CAG TCT TG | AJ507152 |
| Reverse | GAA CCG CCG GTC CAG AAT GTA CA |
| Gene1 . | Sequence (5′-3′) . | . | Accession no. . |
|---|---|---|---|
| MyHC I2 | Forward | GGC CCC TTC CAG CTT GA | L10129 |
| Reverse | TGG CTG CGC CTT GGT TT | ||
| MyHC IIa2 | Forward | TTA AAA AGC TCC AAG AAC TGT TTC A | U11772 |
| Reverse | CCA TTT CCT GGT CGG AAC TC | ||
| MyHC IIx2 | Forward | TGC TTC AAG TTC TGC CCC ACT | U90719 |
| Reverse | GGC TGC GGG TTA TTG ATG G | ||
| MyHC IIb2 | Forward | CAC TTT AAG TAG TTG TCT GCC TTG | U90720 |
| Reverse | AG GGC AGC AGG GCA CTA GAT GT | ||
| β-Actin | Forward | GGC ATC CAC GAG ACC ACC TTC AA | U07786 |
| Reverse | CAG ACA GCA CCG TGT TGG CGT AGA | ||
| Citrate synthase | Forward | TGC CAG TGC TTC TTC CAC GAA CTT | NM_214276 |
| Reverse | GTT GCC GTG TTG CTG CCT GAA G | ||
| PPARα | Forward | CAA GGG CTT CTT TCG GAG AAC CAT | AF175309 |
| Reverse | GCG CCC AAA TCG AAT TGC ATT AT | ||
| LDH | Forward | GTG CAT CCG ATT TCC ACC ATG ATT | U07178 |
| Reverse | CCA TTC TGT CCC AAG ATG CAA GGA | ||
| Glycogen synthase | Forward | CCC AGT GGG AGG AGG CAG TCT TG | AJ507152 |
| Reverse | GAA CCG CCG GTC CAG AAT GTA CA |
1MyHC = myosin heavy chain; PPARα = peroxisome proliferator-activated receptor α; LDH = lactate dehydrogenase.
2da Costa et al., 2002.
Nucleotide sequences of the primers used for quantitative reverse transcription-PCR
| Gene1 . | Sequence (5′-3′) . | . | Accession no. . |
|---|---|---|---|
| MyHC I2 | Forward | GGC CCC TTC CAG CTT GA | L10129 |
| Reverse | TGG CTG CGC CTT GGT TT | ||
| MyHC IIa2 | Forward | TTA AAA AGC TCC AAG AAC TGT TTC A | U11772 |
| Reverse | CCA TTT CCT GGT CGG AAC TC | ||
| MyHC IIx2 | Forward | TGC TTC AAG TTC TGC CCC ACT | U90719 |
| Reverse | GGC TGC GGG TTA TTG ATG G | ||
| MyHC IIb2 | Forward | CAC TTT AAG TAG TTG TCT GCC TTG | U90720 |
| Reverse | AG GGC AGC AGG GCA CTA GAT GT | ||
| β-Actin | Forward | GGC ATC CAC GAG ACC ACC TTC AA | U07786 |
| Reverse | CAG ACA GCA CCG TGT TGG CGT AGA | ||
| Citrate synthase | Forward | TGC CAG TGC TTC TTC CAC GAA CTT | NM_214276 |
| Reverse | GTT GCC GTG TTG CTG CCT GAA G | ||
| PPARα | Forward | CAA GGG CTT CTT TCG GAG AAC CAT | AF175309 |
| Reverse | GCG CCC AAA TCG AAT TGC ATT AT | ||
| LDH | Forward | GTG CAT CCG ATT TCC ACC ATG ATT | U07178 |
| Reverse | CCA TTC TGT CCC AAG ATG CAA GGA | ||
| Glycogen synthase | Forward | CCC AGT GGG AGG AGG CAG TCT TG | AJ507152 |
| Reverse | GAA CCG CCG GTC CAG AAT GTA CA |
| Gene1 . | Sequence (5′-3′) . | . | Accession no. . |
|---|---|---|---|
| MyHC I2 | Forward | GGC CCC TTC CAG CTT GA | L10129 |
| Reverse | TGG CTG CGC CTT GGT TT | ||
| MyHC IIa2 | Forward | TTA AAA AGC TCC AAG AAC TGT TTC A | U11772 |
| Reverse | CCA TTT CCT GGT CGG AAC TC | ||
| MyHC IIx2 | Forward | TGC TTC AAG TTC TGC CCC ACT | U90719 |
| Reverse | GGC TGC GGG TTA TTG ATG G | ||
| MyHC IIb2 | Forward | CAC TTT AAG TAG TTG TCT GCC TTG | U90720 |
| Reverse | AG GGC AGC AGG GCA CTA GAT GT | ||
| β-Actin | Forward | GGC ATC CAC GAG ACC ACC TTC AA | U07786 |
| Reverse | CAG ACA GCA CCG TGT TGG CGT AGA | ||
| Citrate synthase | Forward | TGC CAG TGC TTC TTC CAC GAA CTT | NM_214276 |
| Reverse | GTT GCC GTG TTG CTG CCT GAA G | ||
| PPARα | Forward | CAA GGG CTT CTT TCG GAG AAC CAT | AF175309 |
| Reverse | GCG CCC AAA TCG AAT TGC ATT AT | ||
| LDH | Forward | GTG CAT CCG ATT TCC ACC ATG ATT | U07178 |
| Reverse | CCA TTC TGT CCC AAG ATG CAA GGA | ||
| Glycogen synthase | Forward | CCC AGT GGG AGG AGG CAG TCT TG | AJ507152 |
| Reverse | GAA CCG CCG GTC CAG AAT GTA CA |
1MyHC = myosin heavy chain; PPARα = peroxisome proliferator-activated receptor α; LDH = lactate dehydrogenase.
2da Costa et al., 2002.
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