Table 2.
Open in new tab

Primer sequences utilized for fecal and ruminal samples during bacterial tag-encoded FLX amplicon pyrosequencing

NamePrimer sequence (5′–3′)
454-F30GCCTCCCTCGCGCCATCAGCGCACTACGTGTGCCAGCMGCNGCGG
454-F31GCCTCCCTCGCGCCATCAGCGCAGCTGTTGTGCCAGCMGCNGCGG
454-F32GCCTCCCTCGCGCCATCAGCGCATACAGTGTGCCAGCMGCNGCGG
454-F33GCCTCCCTCGCGCCATCAGCGCATCTATAGTGCCAGCMGCNGCGG
454-F34GCCTCCCTCGCGCCATCAGCGCATTGGTGGTGCCAGCMGCNGCGG
454-F35GCCTCCCTCGCGCCATCAGCGCCAGAAAAGTGCCAGCMGCNGCGG
454-F36GCCTCCCTCGCGCCATCAGTGTGACGTACGTGCCAGCMGCNGCGG
454-F37GCCTCCCTCGCGCCATCAGTGTGTGCATAGTGCCAGCMGCNGCGG
454-F38GCCTCCCTCGCGCCATCAGTGTGTCCTCAGTGCCAGCMGCNGCGG
454-F39GCCTCCCTCGCGCCATCAGTGTGCATCACGTGCCAGCMGCNGCGG
454-F40GCCTCCCTCGCGCCATCAGTGTGCCTAGAGTGCCAGCMGCNGCGG
454-F41GCCTCCCTCGCGCCATCAGTGTACATAGTGTGCCAGCMGCNGCGG
454-F42GCCTCCCTCGCGCCATCAGTGTACATTGAGTGCCAGCMGCNGCGG
454-F43GCCTCCCTCGCGCCATCAGTGTACATTGTGTGCCAGCMGCNGCGG
454-F44GCCTCCCTCGCGCCATCAGTGTACCAACAGTGCCAGCMGCNGCGG
454-F45GCCTCCCTCGCGCCATCAGTGTACCAACTGTGCCAGCMGCNGCGG
454-F46GCCTCCCTCGCGCCATCAGTGTACCAATCGTGCCAGCMGCNGCGG
454-F47GCCTCCCTCGCGCCATCAGTGTACCAGATGTGCCAGCMGCNGCGG
454-F48GCCTCCCTCGCGCCATCAGTGTACCCATAGTGCCAGCMGCNGCGG
454-F49GCCTCCCTCGCGCCATCAGTGTACAGGGTGTGCCAGCMGCNGCGG
454-F50GCCTCCCTCGCGCCATCAGTGTACCTATCGTGCCAGCMGCNGCGG
linkerB-1100RGCCTTGCCAGCCCGCTCAGGGGTTNCGNTCGTTG
NamePrimer sequence (5′–3′)
454-F30GCCTCCCTCGCGCCATCAGCGCACTACGTGTGCCAGCMGCNGCGG
454-F31GCCTCCCTCGCGCCATCAGCGCAGCTGTTGTGCCAGCMGCNGCGG
454-F32GCCTCCCTCGCGCCATCAGCGCATACAGTGTGCCAGCMGCNGCGG
454-F33GCCTCCCTCGCGCCATCAGCGCATCTATAGTGCCAGCMGCNGCGG
454-F34GCCTCCCTCGCGCCATCAGCGCATTGGTGGTGCCAGCMGCNGCGG
454-F35GCCTCCCTCGCGCCATCAGCGCCAGAAAAGTGCCAGCMGCNGCGG
454-F36GCCTCCCTCGCGCCATCAGTGTGACGTACGTGCCAGCMGCNGCGG
454-F37GCCTCCCTCGCGCCATCAGTGTGTGCATAGTGCCAGCMGCNGCGG
454-F38GCCTCCCTCGCGCCATCAGTGTGTCCTCAGTGCCAGCMGCNGCGG
454-F39GCCTCCCTCGCGCCATCAGTGTGCATCACGTGCCAGCMGCNGCGG
454-F40GCCTCCCTCGCGCCATCAGTGTGCCTAGAGTGCCAGCMGCNGCGG
454-F41GCCTCCCTCGCGCCATCAGTGTACATAGTGTGCCAGCMGCNGCGG
454-F42GCCTCCCTCGCGCCATCAGTGTACATTGAGTGCCAGCMGCNGCGG
454-F43GCCTCCCTCGCGCCATCAGTGTACATTGTGTGCCAGCMGCNGCGG
454-F44GCCTCCCTCGCGCCATCAGTGTACCAACAGTGCCAGCMGCNGCGG
454-F45GCCTCCCTCGCGCCATCAGTGTACCAACTGTGCCAGCMGCNGCGG
454-F46GCCTCCCTCGCGCCATCAGTGTACCAATCGTGCCAGCMGCNGCGG
454-F47GCCTCCCTCGCGCCATCAGTGTACCAGATGTGCCAGCMGCNGCGG
454-F48GCCTCCCTCGCGCCATCAGTGTACCCATAGTGCCAGCMGCNGCGG
454-F49GCCTCCCTCGCGCCATCAGTGTACAGGGTGTGCCAGCMGCNGCGG
454-F50GCCTCCCTCGCGCCATCAGTGTACCTATCGTGCCAGCMGCNGCGG
linkerB-1100RGCCTTGCCAGCCCGCTCAGGGGTTNCGNTCGTTG
Table 2.
Open in new tab

Primer sequences utilized for fecal and ruminal samples during bacterial tag-encoded FLX amplicon pyrosequencing

NamePrimer sequence (5′–3′)
454-F30GCCTCCCTCGCGCCATCAGCGCACTACGTGTGCCAGCMGCNGCGG
454-F31GCCTCCCTCGCGCCATCAGCGCAGCTGTTGTGCCAGCMGCNGCGG
454-F32GCCTCCCTCGCGCCATCAGCGCATACAGTGTGCCAGCMGCNGCGG
454-F33GCCTCCCTCGCGCCATCAGCGCATCTATAGTGCCAGCMGCNGCGG
454-F34GCCTCCCTCGCGCCATCAGCGCATTGGTGGTGCCAGCMGCNGCGG
454-F35GCCTCCCTCGCGCCATCAGCGCCAGAAAAGTGCCAGCMGCNGCGG
454-F36GCCTCCCTCGCGCCATCAGTGTGACGTACGTGCCAGCMGCNGCGG
454-F37GCCTCCCTCGCGCCATCAGTGTGTGCATAGTGCCAGCMGCNGCGG
454-F38GCCTCCCTCGCGCCATCAGTGTGTCCTCAGTGCCAGCMGCNGCGG
454-F39GCCTCCCTCGCGCCATCAGTGTGCATCACGTGCCAGCMGCNGCGG
454-F40GCCTCCCTCGCGCCATCAGTGTGCCTAGAGTGCCAGCMGCNGCGG
454-F41GCCTCCCTCGCGCCATCAGTGTACATAGTGTGCCAGCMGCNGCGG
454-F42GCCTCCCTCGCGCCATCAGTGTACATTGAGTGCCAGCMGCNGCGG
454-F43GCCTCCCTCGCGCCATCAGTGTACATTGTGTGCCAGCMGCNGCGG
454-F44GCCTCCCTCGCGCCATCAGTGTACCAACAGTGCCAGCMGCNGCGG
454-F45GCCTCCCTCGCGCCATCAGTGTACCAACTGTGCCAGCMGCNGCGG
454-F46GCCTCCCTCGCGCCATCAGTGTACCAATCGTGCCAGCMGCNGCGG
454-F47GCCTCCCTCGCGCCATCAGTGTACCAGATGTGCCAGCMGCNGCGG
454-F48GCCTCCCTCGCGCCATCAGTGTACCCATAGTGCCAGCMGCNGCGG
454-F49GCCTCCCTCGCGCCATCAGTGTACAGGGTGTGCCAGCMGCNGCGG
454-F50GCCTCCCTCGCGCCATCAGTGTACCTATCGTGCCAGCMGCNGCGG
linkerB-1100RGCCTTGCCAGCCCGCTCAGGGGTTNCGNTCGTTG
NamePrimer sequence (5′–3′)
454-F30GCCTCCCTCGCGCCATCAGCGCACTACGTGTGCCAGCMGCNGCGG
454-F31GCCTCCCTCGCGCCATCAGCGCAGCTGTTGTGCCAGCMGCNGCGG
454-F32GCCTCCCTCGCGCCATCAGCGCATACAGTGTGCCAGCMGCNGCGG
454-F33GCCTCCCTCGCGCCATCAGCGCATCTATAGTGCCAGCMGCNGCGG
454-F34GCCTCCCTCGCGCCATCAGCGCATTGGTGGTGCCAGCMGCNGCGG
454-F35GCCTCCCTCGCGCCATCAGCGCCAGAAAAGTGCCAGCMGCNGCGG
454-F36GCCTCCCTCGCGCCATCAGTGTGACGTACGTGCCAGCMGCNGCGG
454-F37GCCTCCCTCGCGCCATCAGTGTGTGCATAGTGCCAGCMGCNGCGG
454-F38GCCTCCCTCGCGCCATCAGTGTGTCCTCAGTGCCAGCMGCNGCGG
454-F39GCCTCCCTCGCGCCATCAGTGTGCATCACGTGCCAGCMGCNGCGG
454-F40GCCTCCCTCGCGCCATCAGTGTGCCTAGAGTGCCAGCMGCNGCGG
454-F41GCCTCCCTCGCGCCATCAGTGTACATAGTGTGCCAGCMGCNGCGG
454-F42GCCTCCCTCGCGCCATCAGTGTACATTGAGTGCCAGCMGCNGCGG
454-F43GCCTCCCTCGCGCCATCAGTGTACATTGTGTGCCAGCMGCNGCGG
454-F44GCCTCCCTCGCGCCATCAGTGTACCAACAGTGCCAGCMGCNGCGG
454-F45GCCTCCCTCGCGCCATCAGTGTACCAACTGTGCCAGCMGCNGCGG
454-F46GCCTCCCTCGCGCCATCAGTGTACCAATCGTGCCAGCMGCNGCGG
454-F47GCCTCCCTCGCGCCATCAGTGTACCAGATGTGCCAGCMGCNGCGG
454-F48GCCTCCCTCGCGCCATCAGTGTACCCATAGTGCCAGCMGCNGCGG
454-F49GCCTCCCTCGCGCCATCAGTGTACAGGGTGTGCCAGCMGCNGCGG
454-F50GCCTCCCTCGCGCCATCAGTGTACCTATCGTGCCAGCMGCNGCGG
linkerB-1100RGCCTTGCCAGCCCGCTCAGGGGTTNCGNTCGTTG
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