... permissive alternatives might be replaced. ectogenesis embryo destruction human embryo research regulation time limit Various time limits have been proposed for human embryo research, including the original “14-day rule” prohibiting research beyond 14 days...

... and directly compared them with those from peri-implantation human embryos during extended embryo culture (EEC) between embryonic days 8 to 12. The BAP and TSC grouped closely with TB cells from EEC within each TB sublineage following dimensional analysis and unsupervised hierarchical clustering. However...

... embryo viability assessment. Exploiting the well-known capabilities of transfer learning, we illustrate the performance of CNN models for very limited datasets, paving the way for the use on a clinic-by-clinic basis, catering for local data heterogeneity. IVF human embryo assessment blastocyst Machine...

... (ZNF652) transcription factor ( Figs 2C and 3 , Supplementary Table S6 ). uniparental androgenote androcyte haploid human embryo single-cell transcriptomic analysis RNA-seq early embryo development Instituto de Salud Carlos III 10.13039/501100004587 European Regional Development Fund...

... humans and mice is currently unknown. Here, we mapped parental haploid epigenomes using human parthenogenetic and androgenetic embryos. Human embryos have a larger portion of genome with parentally specific epigenetic states than mouse embryos. The allelic patterns of epigenetic states for orthologous...

... Journals Publication Model ( https://dbpia.nl.go.kr/pages/standard-publication-reuse-rights ) Abstract STUDY QUESTION Are the extracellular vesicles (EVs) secreted by the maternal endometrium uptaken by human embryos and is their miRNA cargo involved in implantation and embryo development? SUMMARY...

... trends in PGT practice. European Society of Human Reproduction and Embryology PGT structural rearrangements monogenic disorders aneuploidy human embryo registry data collection comprehensive genetic testing ABSTRACT STUDY QUESTION What are the trends and developments in preimplantation genetic...

... preimplantation development? SUMMARY ANSWER The presence of a pathogenic mitochondrial variant triggers changes in the gene expression of preimplantation human embryos, compromising their development, cell differentiation, and survival. WHAT IS KNOWN ALREADY Quantitative and qualitative anomalies...

... mutations potentially with loss-of-function, gain-of-function, and other unknown consequences. Furthermore, a recent study found that CRISPR-Cas9 administration in early human embryos leads to frequent loss of the targeted chromosome and uncontrollable chromosome rearrangement ( Zuccaro et al., 2020...

... associate (TEA) domain family member 4 (TEAD4) in trophectoderm (TE) differentiation during human embryo preimplantation development in comparison to mouse? SUMMARY ANSWER TEAD4 regulates TE lineage differentiation in the human preimplantation embryo acting upstream of caudal-type...

... Figure 1 31 12 2021 11 4 2022 3 5 2022 31 05 2022 Graphical Abstract Graphical Abstract human embryo stem cells embryoid implantation peri-implantation embryogenesis National Natural Science Foundation of China 10.13039/501100001809 32130034...

..., these findings provide clues for the causes of human embryonic arrest after the eight-cell stage, and they also provide new ideas for improving the success rate of ART in clinical practice. human embryo maternal factor heterogeneity blastocyst formation single-cell RNA sequencing embryonic development Wnt...

... (data not shown). To study the activity of TLR3 and TLR5 in D-5 human embryos, their specific ligands poly (I: C) (InvivoGen, San Diego, CA, USA) and flagellin (FLA-ST Ultrapure, from Salmonellatyphimurium, InvivoGen, San Diego, CA, USA), were added to embryo culture media. Fifteen...

.../A. PGT PGD structural rearrangements monogenic disorders aneuploidy human embryo registry data collection pre-implantation genetic testing The main objectives of the ESHRE PGT Consortium, established in 1997, are to provide guidance and network opportunities to pre-implantation genetic testing...

...Wuwen Zhang; Shifeng Li; Kai Li; L i Li; Ping Yin; Guoqing Tong Previous studies have shown that embryos cultured in vitro are often prone to developmental arrest; cattle and sheep embryos both occur at 8–16 cell stages, and human embryos usually occur at 4–8 cell stages [ 8–10 ]. Early...

.../standard_publication_model ) Abstract STUDY QUESTION What is the role of POU class 5 homeobox 1 (POU5F1) in human preimplantation development and how does it compare with the mouse model? SUMMARY ANSWER POU5F1 is required for successful development of mouse and human embryos to the blastocyst stage...

... INTEREST(S) This work was supported by a research grant from KU Leuven University (OT/14/109). The authors declare no competing financial, professional or personal interests. TRIAL REGISTRATION NUMBER KU Leuven S57378 (ML11309), B322201523225. male age human embryo development ART ELPO study APA...

...(S) The study has no external funding and all costs are covered by ESHRE. There are no competing interests declared. TRIAL REGISTRATION NUMBER N/A. PGT PGD structural rearrangements monogenic disorders aneuploidy human embryo registry data collection preimplantation genetic testing...

...Peter T Ruane; Chelsea J Buck; Phoebe A Babbington; Wedad Aboussahoud; Stéphane C Berneau; Melissa Westwood; Susan J Kimber; John D Aplin; Daniel R Brison Studying human embryo implantation directly requires the use of in vitro models. Interactions between blastocysts and primary human...

... be used for assisted reproductive technology. SC, stem cell; MII, metaphase II; GV, germinal vesicle; iPS, induced pluripotent stem cell; CRISPR, clustered regularly interspaced short palindromic repeats. Table 1 Reported human embryo editing experiment Study Embryo type Gene Off-target...