... differentiation. To uncover the mechanism of PORCN action during chondrogenesis, we used two different PORCN inhibitors, C59 and LGK974, in several model systems, including micromasses, 3D cell cultures, long bone tissue cultures and zebrafish animal model. PORCN inhibitors enhanced cartilaginous extracellular...

...   R , Nishi   S   et al.   Culture-independent method for identification of microbial enzyme-encoding genes by activity-based single-cell sequencing using a water-in-oil microdroplet platform . Sci Rep   2016 ; 6 : 22259 . 10.1038/srep22259 4. Long   AM , Hou   S...

... in Pakistan. The aim of this study was to report the prevalence and antimicrobial resistance patterns of strains of S. Typhi and S. Paratyphi cases using data from large public and private laboratory networks across Pakistan from 2017 to 2019. A large-scale review of blood culture...

... and greenhouse gas emissions. In this study, we explored secondary metabolites produced by methane-oxidizing bacteria (MOB) and their ecological roles. We analyzed the volatile profiles of four MOB strains under controlled conditions and conducted a meta-analysis using high-quality genomes from 62 cultured MOB...

... cultures did not predict eye infections, but certain Candida species and symptoms did. Hospital mortality was linked to factors like age, infection type, and use of vasopressors. candidemia endophthalmitis chorioretinitis blood cultures mortality Abstract Candidemia can lead to ocular...

... to studying right authoritarianism across distinct country contexts—against determinism and toward international resistance. right-wing politics political economy political communication globalization and media cultures comparative analysis The past decade has been marked by the growing electoral...

..., P = 0.14). The IRR between the pre- and post-intervention phase was 0.78 (95% CI 0.74, 0.83, P < 0.01). Table 1: Comparing patient demographics and adverse outcomes between pre-intervention and post-intervention periods Pre-intervention (3439 blood culture events, 893...

... contact [email protected] for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact [email protected]. Abstract Background Blood culture...

... has required technical innovations, both in adapting existing techniques and developing entirely new ones. This review discusses the existing methods used to study eight distinct aspects of cable bacteria research, including the challenges of culturing them in laboratory conditions, performing...

... on the historical antecedents of the culture of evidence, and these views are also reported. Primary data were collected through a series of interviews, during 2020, as part of a wider project on the use of evidence in energy policymaking (reported in McDowall & Britchfield, 2020 ). Informed consent...

..., substantial financial costs, as well as variances to the original tumor. Table 1. Overview of Characteristics of Different Brain Tumor Models Cell lines Sphere stem-like cultures Brain organoids Organotypic brain slice cultures Syngeneic mouse models Human cell line xenografts...

... a promising and practical in vitro alternative to 2D cell line cultures for the early hepatotoxicity screening of drug candidates. microphysiological system DILI drug-induced liver injury drug safety spheroid cultures Corresponding author: InSphero AG , Wagistrasse 27a, CH-8952 Schlieren, Switzerland...

... as a consultant with bioMérieux for rapid pathogen testing. D. J. D. has received research funding from bioMérieux for clinical trials of susceptibility testing technology. All other authors report no potential conflicts. The current blood culture bottle shortage emphasizes the importance of diagnostic...

...Romney M Humphries; Patty W Wright; Ritu Banerjee; Daniel E Dulek; John C Champion; David C Gaston; Thomas R Talbot References 1 Fabre   V , Sharara   SL , Salinas   AB , Carroll   KC , Desai   S , Cosgrove   SE . Does this patient need blood cultures? A scoping review...

...Raja J Hofmeister; Mark D Gonzalez; Jonathan Hildreth; Troy Savage; Amy Leber; Preeti Jaggi In this single-center study [ 6 ], ETT cultures were reduced from 46 to 19 per month, for an average decrease of 27 ETT cultures per month. We assumed that 95% would have been negative and 5% positive...

... associated with intravascular catheters pose significant financial burden, morbidity, and mortality. There is much debate about whether or not blood cultures should be drawn through central venous catheters, and while guidelines advocate for catheter-drawn cultures when catheter infection is suspected...

... and phenotypical (functional) features. Biogeochemical processes in natural and engineered ecosystems are generally performed by more than a single microbe and often rely on mutualistic interactions. We demonstrate the rational bottom-up design of synthetic, interdependent co-cultures to achieve concomitant...

... ago. We are embedded in society so definitions of scope will vary depending upon the dominant ideology and the dominant political approaches, something JCR’s epistemic culture and community aren’t immune to. In such a climate, midrange scholarship often engages with social and environmental...

... fungal communities Jinhua ham Penicillium starter cultures volatile compounds Correspondent: E-mail: [email protected] and [email protected] Abstract To investigate...

... years. We also performed subanalyses for (1) outcome of UTI as defined by the Infectious Diseases Society of America (IDSA), (2) growth of Escherichia coli in urine culture, and (3) clean catch collection methods. During the study period, 220 531 encounters met study criteria. After using...