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Mikio Nishimura, Parichart Bhusawang, Kazimierz Strzalka, Takashi Akazawa, Developmental Formation of Glutamine Synthetase in Greening Pumpkin Cotyledons and Its Subcellular Localization , Plant Physiology, Volume 70, Issue 2, August 1982, Pages 353–356, https://doi.org/10.1104/pp.70.2.353
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Abstract
During the germination of pumpkin (Cucurbita sp. Amakuri Nankin) seeds in dark, the activity of glutamine synthetase in cotyledons gradually increased, reaching a maximum at 5 to 6 days. A measurable enhancement (about 4-fold) of the enzyme activity occurred when the seedlings were exposed to continuous illumination from day 4 up to day 8. Glutamine synthetase activity was detectable only in the cytosolic fraction in the etiolated cotyledons, whereas it was found both in the cytosolic and chloroplast fractions in the green cotyledons. The two isoenzymes of glutamine synthetase have been separated by DEAE-cellulose column chromatography of extracts from the green cotyledons. These data indicate that during the greening process the chloroplastic glutamine synthetase is newly synthesized. The roles of cytosolic and chloroplastic glutamine synthetase in germinating pumpkin cotyledons concerning assimilation of NH3 are discussed.
Visiting scientist sponsored by the Japan Society for the Promotion of Science (JSPS) (1980). Present address: Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 5, Thailand.
Visiting scientist sponsored by the Ministry of Education, Science and Culture (Mombusho) (1980). Present address: Institute of Molecular Biology, Jagellonian University, Krakow, Poland.
This is paper 58 in the series of “Structure and Function of Chloroplast Proteins.” Supported in part by the research grants (No. 511606, 511806) from the Ministry of Education, Science and Culture (Mombusho).
