Volume 37, Issue 4, April 2025
Editorial
Embracing the challenges ahead: a new chapter for the plant cell leadership
In Brief
Living off the fat of the plant: Powdery mildew fungi thrive on host thylakoid lipids
Bending over backwards for soil emergence: KIPK and KIPK-LIKE1 regulate hypocotyl gravitropic growth
Dawning aurora: TPXL3 activates α-Aurora kinase and regulates mitotic spindle morphogenesis
The complex immune puzzle: A deeper dive into the MORC1-mediated broad-spectrum defense signaling pathway
Palmer amaranth's arsenal: Rearrangement of eccDNA provides dual herbicide resistance in Amaranthus palmeri
Flipping the sex switch: Genetic insights into sex determination factor in Ceratopteris richardii
Using chimeric rice proteins to make heads or tails of the function of repetitive elements in Gγ subunits
Like Alice in Wonderland, ROOT-ExM enlarges root tips for a closer look
How UV-B-activated UVR8 triggers LOX1 to close stomata: Unveiling a mechanism of photoreceptor-regulated enzyme activity in the cytoplasm
Letter to the Editor
Seeing is believing: Whole-cell electron tomography models of vacuole morphology and formation in the early-stage root cortex of Arabidopsis
Breakthrough Reports
Root expansion microscopy: A robust method for super resolution imaging in Arabidopsis
Root expansion microscopy (ROOT-ExM) achieves super-resolution expansion microscopy in plants.
Super-resolution expansion microscopy in plant roots
The development of expansion microscopy in the Arabidopsis root enabled the imaging of molecular targets in tissues at diffraction-unlimited resolution.
Large-Scale Biology
Systems analysis of long-term heat stress responses in the C4 grass Setaria viridis
Long-term heat stress does not affect photosynthesis in the C4 grass Setaria viridis owing to an effective acclimation response of carbon assimilation, although it inhibits plant growth.
A comprehensive all-in-one CRISPR toolbox for large-scale screens in plants
An all-in-one CRISPR toolbox is developed to aid guide RNA library-based loss-of-function and gain-of-function screens in plants.
Research Articles
The kinesin motor POS3 and the microtubule polymerase MOR1 coordinate chromosome congression during mitosis in Arabidopsis
The kinesin-like protein POS3 dynamically associates with kinetochores and facilitates chromosome alignment in coordination with the microtubule polymerase MOR1 during mitosis.
A CRY1–HY5–MYB signaling cascade fine-tunes guard cell reactive oxygen species levels and triggers stomatal opening
The Arabidopsis blue-light receptor CRY1 and its downstream HY5 protein optimize daytime stomatal conductance through spatiotemporal control of flavonols and ROS homeostasis in guard cells.
The UV-B photoreceptor UVR8 interacts with the LOX1 enzyme to promote stomatal closure through the LOX-derived oxylipin pathway
A noncanonical signaling module consisting of a UV-B photoreceptor and a cytoplasmic lipoxygenase mediates stomatal responses to UV-B light.
Genetic and transcriptome analyses of the effect of genotype-by-environment interactions on Brassica napus seed oil content
A systematic investigation of genotype-by-environment interactions for seed oil content in rapeseed revealed that BnaA02.LSH5 affects seed oil accumulation in response to light intensity.
The kinase ATM delays Arabidopsis leaf senescence by stabilizing the phosphatase MKP2 in a phosphorylation-dependent manner
A kinase delays Arabidopsis leaf senescence by stabilizing a phosphatase under oxidative stress, and the aging-related module may be conserved across eukaryotes.
LTD coordinates chlorophyll biosynthesis and LIGHT-HARVESTING CHLOROPHYLL A/B-BINDING PROTEIN transport
LTD couples chlorophyll biosynthesis with light-harvesting chlorophyll a/b-binding protein transport, maintaining their stoichiometric ratio to ensure the assembly of the light-harvesting complex.
The G-protein β subunit SlGB1 regulates tyramine-derived phenolamide metabolism for shoot apex growth and development in tomato
Tomato plants have a tyramine-derived phenolamide metabolic pathway regulated by the G-protein β subunit that is absent in Arabidopsis, which is essential for shoot apex growth and development.
ZmSSRP1 facilitates the progression of RNA polymerase II and is essential for kernel development in maize
ZmSSRP1, a subunit of the FACT histone chaperone complex, is required for RNA Polymerase II progression during transcription in maize kernel development.
The psbA open reading frame acts in cis to toggle HCF173 from an activator to a repressor for light-regulated psbA translation in plants
Light activates psbA translation for photosystem II repair in plants by relieving repression by the psbA ORF, toggling a translational regulator bound to psbA mRNA from a repressor to an activator.
A rearranged Amaranthus palmeri extrachromosomal circular DNA confers resistance to glyphosate and glufosinate
The unconventional TPX2 family protein TPXL3 regulates α Aurora kinase function in spindle morphogenesis in Arabidopsis
TPXL3 targets α-Aurora kinase to mitotic microtubule arrays and activates its function in bipolar spindle assembly.
The protein kinases KIPK and KIPK-LIKE1 suppress overbending during negative hypocotyl gravitropic growth in Arabidopsis
Protein kinases are essential for enabling seedlings to grow against gravity, potentially by facilitating the appropriate distribution of auxin throughout the growing seedling hypocotyl.
The circular RNA circANK suppresses rice resistance to bacterial blight by inhibiting microRNA398b-mediated defense
Specific knockdown of the circular RNA Os-circANK reveals its function as a negative regulator of microRNA398b-mediated resistance to bacterial blight in rice.
The transcription factors DOF4.6 and XND1 jointly regulate root hydraulics and drought responses in Arabidopsis
A DOF transcription factor interacts with a xylem-specific transcription factor, affecting root hydraulics and drought stress responses via combined effects on xylem formation and aquaporin functions.
A deficient CP24 allele defines variation for dynamic nonphotochemical quenching and photosystem II efficiency in maize
High throughput phenotyping of a maize multi-founder mapping population reveals genetic factors controlling dynamic photoprotection and photosystem II efficiency in maize.