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J.W. Hofstraat, K. Rubelowsky, S. Slutter, Corrected fluorescence excitation and emission spectra of phytoplankton: toward a more uniform approach to fluorescence measurements, Journal of Plankton Research, Volume 14, Issue 5, 1992, Pages 625–636, https://doi.org/10.1093/plankt/14.5.625
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Abstract
Techniques for correction of fluorescence emission and excitation spectra of phytoplankton are described, which can be applied in any commercially available spectrophotometer. The correction of the emission spectrum is based on the measurement of a calibrated light source. The excitation spectra are corrected by means of a quantum counter solution that measures the spectral intensity of the excitation system and separate correction for wavelength-dependent effects of the excitation optics. The correction procedures give technically corrected spectra, i.e. spectra that are free from wavelength dependent bias, but do not give absolute intensity values. Spectra that have been properly corrected for instrumental wavelength dependencies are suitable for intercomparison, both intra- and interlaboratory. Another application is the derivation of spectral data that will be obtained by other techniques that make use of fluorescence measurements, such as flow cytometry, remote sensing and in situ instruments. A necessary condition is that the spectral response functions of these instruments must be known.
