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Xiufeng Wu, Richard Yuan, Michael Bacica, Stephen J Demarest, Generation of orthogonal Fab-based trispecific antibody formats, Protein Engineering, Design and Selection, Volume 31, Issue 7-8, July-August 2018, Pages 249–256, https://doi.org/10.1093/protein/gzy007
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Abstract
The clinical success of monoclonal antibodies to treat diseases across nearly every therapeutic area has spurred advances in bispecific antibody technology with the goal of cost-effectively combining various therapies or providing novel mechanisms for disease intervention. Many novel bispecific antibodies are now in clinical development or the late pre-clinical setting. A new horizon exists for novel molecular entities with the ability to bind three or more antigens. Here we describe the production and characterization of novel trispecific antibody-like proteins denoted ‘OrthoTsAbs’ that self-assemble through the application of engineered antibody domain interfaces.