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NOBUMARO KAWASHIMA, AYAKO IMAI, EINOSUKE TAMAKI, STUDIES ON PROTEIN METABOLISM IN HIGHER PLANTS. III. CHANGES IN THE SOLUBLE PROTEIN COMPONENTS WITH LEAF GROWTH, Plant and Cell Physiology, Volume 8, Issue 3, October 1967, Pages 447–458, https://doi.org/10.1093/oxfordjournals.pcp.a079279
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Abstract
Water soluble protein was extracted from tobacco leaves (Bright Yellow) and fractionated through chromatographic and immunochemical procedures. Six different UV-absorbing components, 7 antigenic components, and 4 enzyme activities (phosphatase, protease, peroxidase and RNase) were detected on the Sephadex chromatograms of leaf extracts. An UV-absorbing fraction, Fr. I-2, (immunochemically designated as Pr. Imm-I) corresponded to the known “Fraction I ” of S. G. WILDMAN. The contents of the three antigenic components, Imm-a, Imm-b and Imm-c, having estimated molecular weights of 1 to 2 × 105, showed significant fluctuations during growth of the leaves. Peroxidase and another antigenic component (Imm-f) of smaller molecular weight showed increase with age of the leaves. Properties of the protein components thus detected were investigated.