Abstract

Water soluble protein was extracted from tobacco leaves (Bright Yellow) and fractionated through chromatographic and immunochemical procedures. Six different UV-absorbing components, 7 antigenic components, and 4 enzyme activities (phosphatase, protease, peroxidase and RNase) were detected on the Sephadex chromatograms of leaf extracts. An UV-absorbing fraction, Fr. I-2, (immunochemically designated as Pr. Imm-I) corresponded to the known “Fraction I ” of S. G. WILDMAN. The contents of the three antigenic components, Imm-a, Imm-b and Imm-c, having estimated molecular weights of 1 to 2 × 105, showed significant fluctuations during growth of the leaves. Peroxidase and another antigenic component (Imm-f) of smaller molecular weight showed increase with age of the leaves. Properties of the protein components thus detected were investigated.

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