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K. YOSHIDA, K. UMEMURA, K. YOSHINAGA, Y. OOTA, SPECIFIC RNA FROM PHOTOPERIODICALLY INDUCED COTYLEDONS OF PHARBITIS NIL, Plant and Cell Physiology, Volume 8, Issue 1, March 1967, Pages 97–108, https://doi.org/10.1093/oxfordjournals.pcp.a079255
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Abstract
The nucleotide ratio of several RNA species from cotyledons of Pharbitis nil subjected to a single 16 hr night with or without a 15 min light-break, or to continuous light was investigated. RNA species examined were RNAs from nuclear, mitochondrial, microsomal, and supernatant fractions separated by differential centrifugation, and s-, r-, and m-RNAs fractionated by methylated albumin column chromatography.
Of the RNAs examined, m-RNA alone was found to change its nucleotide ratio with photoperiod applied. Thus as compared with m-RNA from non-induced cotyledons (exposed to continuous light or an interrupted night), m-RNA from cotyledons induced by an uninterrupted night contained significantly reduced guanylic and cytidylic acids on molar ratio basis.
A working hypothesis was proposed that floral stimulus production in cotyledons may be directed by gene DNA derepressed photoperiodically.