Abstract

We investigated the promoter region of atx1, which encodes a copper chaperone in response to iron deficiency induction. Deletion analysis of the promoter region from the 5′ and 3′ ends revealed that the –532/–461 and –320/–276 regions were necessary and sufficient for iron deficiency-inducible expression. Further deletion analysis showed that two of the Fe deficiency-responsive elements (FeREs) localized within the –532/–511 and –306/–276 regions, in which AtxFeRE1 at –529/–515 (GTCGCACTGGCATGT) and AtxFeRE2 at –300/–286 (GCAGCGATGGCATTT) had been identified, respectively, with a conserved sequence of GNNGCNNTGGCATNT, differing from all known FeREs found in other organisms.

You do not currently have access to this article.