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Xiaowen Fei, Xiaodong Deng, A Novel Fe Deficiency-Responsive Element (FeRE) Regulates the Expression of atx1 in Chlamydomonas reinharditii, Plant and Cell Physiology, Volume 48, Issue 10, October 2007, Pages 1496–1503, https://doi.org/10.1093/pcp/pcm110
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Abstract
We investigated the promoter region of atx1, which encodes a copper chaperone in response to iron deficiency induction. Deletion analysis of the promoter region from the 5′ and 3′ ends revealed that the –532/–461 and –320/–276 regions were necessary and sufficient for iron deficiency-inducible expression. Further deletion analysis showed that two of the Fe deficiency-responsive elements (FeREs) localized within the –532/–511 and –306/–276 regions, in which AtxFeRE1 at –529/–515 (GTCGCACTGGCATGT) and AtxFeRE2 at –300/–286 (GCAGCGATGGCATTT) had been identified, respectively, with a conserved sequence of GNNGCNNTGGCATNT, differing from all known FeREs found in other organisms.