Abstract

Localization of xyloglucan in cell walls regenerated from tobacco protoplasts (Nicotiana tabacum L.; cv. BY-2) is visualized by rapid-freezing and deep-etching (RFDE) electron microscopy coupled with immunogold electron microscopy. Xyloglucan was already deposited in the cell wall 3 h after culture initiation. Xyloglucan was mainly localized along microfibrils with a lesser amount in intersections between two crossed microfibrils in 120-hour-old cells. These data support the previous hypothesis of Keegstra et al. (1973) that propose an interconnection between xyloglucan and cellulose.

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