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Munehiro Kikuyama, Teruo Shimmen, Role of Ca2+ on Triggering Tonoplast Action Potential in Intact Nitella flexilis, Plant and Cell Physiology, Volume 38, Issue 8, 1997, Pages 941–944, https://doi.org/10.1093/oxfordjournals.pcp.a029255
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Abstract
Sensitive aequorin was microinjected into the cytoplasm of Nitella flexilis to study the role of Ca2+ in the generation of the tonoplast action potential. The temporal relation between the increase in cytoplasmic Ca2+ and the tonoplast action potential suggested that tonoplast action potential is triggered by an increase in cytoplasmic Ca2+. This is also supported by the fact that Mn2+, extracellularly applied, inhibited both the increase in cytoplasmic Ca2+ and the generation of the tonoplast action potential.