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Hiro-Yuki Hirano, Noriko Tabayashi, Takeshi Matsumura, Masatoshi Tanida, Yoshibumi Komeda, Yoshio Sano, Tissue-Dependent Expression of the Rice wx+ Gene Promoter in Transgenic Rice and Petunia, Plant and Cell Physiology, Volume 36, Issue 1, January 1995, Pages 37–44, https://doi.org/10.1093/oxfordjournals.pcp.a078742
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Abstract
The waxy (wx) locus, which controls the amylose synthesis, is known to be expressed specifically in the endosperm and pollen. To study the tissue-specific regulation of the wx+ gene, we introduced a fusion gene that consisted of the upstream sequence of the wx+ gene and the gene for rβ-glucuronidase (GUS) into cells of rice (Oryza sativa L.) and petunia (Petunia hybrida L.). GUS activity was examined in the regenerated transgenic rice and petunia plants. In transgenic rice, the upstream sequence of the wx+ gene was sufficient to direct the tissue-specific expression of GUS in the endosperm and pollen, and the control of expression was quantitative. By contrast, in transgenic petunia, the same fusion gene was expressed in pollen but not in the endosperm. These results suggest that the putative cis-acting elements that direct pollen-specific expression are common to or similar in both monocotyledonous and dicotyledonous plants, whereas ciy-elements responsible for the endosperm-specific expression of the rice wx+ gene do not function in petunia, in which development of the endosperm differs from that in rice.
