Abstract

The cellular level of cyclic 3′,5′-AMP (cAMP) in the cyanobacterium Anabaena cylindrica changed transiently in response to changes in extracellular environments. When the cells were transfered from dark to light, or anaerobic to aerobic conditions in the dark, the cAMP level rapidly decreased within one min and then gradually recovered. Addition of carbonyl cyanide m-chlorophenylhydrazone (CCCP) which inhibits ATP synthesis caused an increase in cAMP level in the light but not in the dark. The level of cAMP increased several fold by lowering the pH from 8 to 6. On the contrary, a rise of pH from 6 to 8 caused a decrease in the cAMP level. It is suggested that the change in membrane electrochemical potential is involved in the regulation of cellular cAMP concentration.

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