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Kiyoshi Tazaki, Naoto Shibuya, Purification and Partial Characterization of a Lectin from the Bark of Japanese Elderberry (Sambucus sieboldiana), Plant and Cell Physiology, Volume 30, Issue 6, September 1989, Pages 899–903, https://doi.org/10.1093/oxfordjournals.pcp.a077822
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Abstract
A lectin [Sambucus sieboldiana agglutinin (SSA)] was purified from the twigs of Sambucus sieboldiana by repeated affinity chromatography on fetuin-Sepharose. SSA had a molecular weight (Mr) of approximately 160 K on gel filtration and consisted of two types of subunit of which the molecular weights ranged from 31 to 37 K. SSA agglutinated human erythrocytes irrespective of their blood type and the hemagglutination was inhibited by a very low concentration of Neu5Ac(α2-6)lactose, suggesting that SSA has a carbohydrate-binding specificity similar to that of the lectin previously isolated from the bark of S. nigra (SNA). However, the Ouchterlony double-diffusion analysis of these lectins with an antibody raised against SSA showed that SSA was immunologjcally related but not identical to SNA.