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Isao Enami, Mariko Kura-Hotta, Effect of Intracellular ATP Levels on the Light-Induced H+ Efflux from Intact Cells of Cyanidium caldarium, Plant and Cell Physiology, Volume 25, Issue 7, October 1984, Pages 1107–1113, https://doi.org/10.1093/oxfordjournals.pcp.a076817
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Abstract
The light-induced H+ efflux observed at acidic pH in Cyanidium cells was shown to be an active H+ transport depending on the intracellular ATP produced by cyclic photo-phosphorylation. Triton X-100 was found to act as an effective uncoupler in intact Cyanidium cells without collapsing the pH gradient across the plasma membrane. Triton X-100 at 0.015% significantly reduced the intracellular ATP levels, stimulated the p-BQ, Hill reaction and completely inhibited the light-induced H+ efflux. Inhibition of the H+ efflux by Triton X-100 correlated well with the depression of the apparent rale of light-induced ATP synthesis as well as the decrease in the intracellular ATP level in light.
The light-induced H+ efflux was completely inhibited by diethylstilbestrol, a specific inhibitor of plasma membrane ATPase, without any changes in the intracellular ATP level, thereby suggesting the participation of the plasma membrane ATPase in the light-induced H+ efflux.
