-
Views
-
Cite
Cite
Tatsuo Omata, Norio Murata, Isolation and Characterization of the Cytoplasmic Membranes from the Blue-Green Alga (Cyanobacterium) Anacystis nidulans, Plant and Cell Physiology, Volume 24, Issue 6, September 1983, Pages 1101–1112, https://doi.org/10.1093/oxfordjournals.pcp.a076614
Close - Share Icon Share
Abstract
Cells of the blue-green alga (cyanobacterium) Anacyslis nidulans were disintegrated, and their thylakoid membranes and cytoplasmic membranes were isolated by floatation centrifugation on a sucrose density gradient. Electron micrographs revealed that the cytoplasmic membranes formed single closed vesicles having diameters of 200–400 nm. These membranes contained xanthophylls as the major constituent pigments and rβ-carotene and chlorophyll a as very minor ones. The major peaks in their absorption spectra were due to carotenoids at 435, 455 and 487 nm, with a minor one due to chlorophyll a at 673 nm. These findings are consistent with the yellow color of the cytoplasmic membranes. The absorption spectrum of the membranes in the carotenoid region was markedly affected by temperature: with a decrease in temperature, the peaks at 455 and 487 nm diminished and a new peak appeared at 390 nm.
