Abstract

The flash-induced 515 nm change in subchloroplast preparations highly enriched in PS II reaction-center components was characterized by a spikelike, brief absorbance change, followed by a small, slow dark-decay process. A comparison with the 515 nm change in class C chloroplasts showed that the spikelike, brief 515 nm change appeared as a result of acceleration of the rapid phase in the dark-decay processes. When an electron donor (diphenylcarbazide) and acceptor (dichlorophenol indophenol) were added together, or when the subchloroplast preparation was embedded in liposomes, a 515 nm change similar to that of class C chloroplasts took the place of the characteristic, spikelike 515 nm change. These results are explained and the difference between the spikelike 515 nm change and the very fast 515 nm changes due to formation of triplet states of carotenoids is discussed.

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