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Eiji Hirasawa, Eiichi Takahashi, Hideaki Matsumoto, Association of nucleotide-phosphohydrolyzing activity with chromatin in germinated pea cotyledon, Plant and Cell Physiology, Volume 20, Issue 1, February 1979, Pages 219–224, https://doi.org/10.1093/oxfordjournals.pcp.a075795
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Abstract
The association of nucleotide-phosphohydrolyzing activity with chromatin in germinated Alaska pea cotyledon was demonstrated by release of activity with DNase treatment and differential elution pattern of the activity of associated and partially dissociated chromatin through a Sephadex G-200 column. The degradation activity for 4 different nucleosidetriphosphates was the highest for GTP, a little lower for CTP and UTP, and least for ATP in the Sephadex G-200 fraction purified from non-histone proteins. The stability of the activity to heat treatment fell after its dissociation from the chromatin structure. The present results support our previous finding that the activity is some kind of non-histone protein and that it reduced RNA synthesizing capacity of in vitro chromatin due to the degradation of substrates.