-
Views
-
Cite
Cite
G. J. T. Frith, K. H. J. Gordon, M. J. Dalling, Proteolytic enzymes in green wheat leaves I. Isolation on DEAE-cellulose of several proteinases with acid pH optima, Plant and Cell Physiology, Volume 19, Issue 3, May 1978, Pages 491–500, https://doi.org/10.1093/oxfordjournals.pcp.a075618
- Share Icon Share
Abstract
Several peptide hydrolases have been isolated in a partially purified form from green wheat leaves by a combination of gel chromatography and chromatography on DEAE-cellulose. These hydrolases all degrade haemoglobin and other substrates tested at an acid pH. Three of the enzymes have been identified as proteinases by measurement of the total N: α-amino N ratio of the degradation products. These three enzymes have a similar optimum assay pH of about 4.5 with haemoglobin as substrate, but differ markedly in their stability at high temperatures.