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Sayoko Mihara, Eiji Hase, Studies on the vegetative life cycle of Chlamydomonas reinhardi Dangeard in synchronous culture IV. Effects of 6-methyl purine on the revolution of the cell cycle, Plant and Cell Physiology, Volume 16, Issue 6, December 1975, Pages 1121–1130, https://doi.org/10.1093/oxfordjournals.pcp.a075231
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Abstract
Cells of Chlamydomonas reinhardi Dangeard were synchronously grown under a 12 hr light-12 hr dark regime. The algal cell cycle under these conditions starts with a light-induced reaction(s) at the beginning of the light period and ends, after a definite period of time (23–24 hr at 25°C), in zoospore liberation. When cells were exposed to 6-methyl purine for short periods (0.5–2.5 hr) at different times during the early and intermediate phases of the cell cycle, it exerted, as an analogue of adenine, two different effects on the revolution of the cell cycle: one a “lengthening” effect seen at its low concentrations in which the length of the cell cycle was somewhat prolonged, the other a “return to start” effect at higher concentrations. In the latter a short exposure of cells to 6-methyl purine brought them to the starting point of the cell cycle concurrent with the abortion of the cycle in process. When 6-methyl purine was applied during the later phase of about 1/4 the length of the cell cycle, it casued no effect. Control of the revolution of the algal cell cycle by an “adenine-involving reaction(s)” disturbed by this adenine analogue is discussed.
