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Karen Y. Fung, George R. Douglas, Daniel Krewski, Statistical analysis of lacZ mutant frequency data from MutaTMMouse mutagenicity assays, Mutagenesis, Volume 13, Issue 3, May 1998, Pages 249–255, https://doi.org/10.1093/mutage/13.3.249
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Abstract
Transgenic mouse assays have provided an unprecedented opportunity to study mutagenesis in diverse rodent tissues. In this article data from MutaTMMouse mutagenicity assays based on the Escherichia coli gene lacZ were analyzed systematically using liver and bone marrow as potential target tissues. Sources of variation, including plates (within ackaging reactions), packaging reactions (within animals) and animals, were evaluated for extrabinomial variation. Although hardly any evidence of overdispersion was detected at the plate level, limited evidence of extra-binomial variation was observed at the packaging reaction level. There was, however, much stronger evidence of overdispersion at the animal level.Statistical tests for increasing trend in mutant frequency with Increasing dose were also performed at the animal level. A significant increasing trend following exposure to /V-nitrosodibenzylamine was detected in liver but not in bone marrow. A logistical model was used to further describe the dose-response relationship observed hi nitrosodibenzylamine-treated liver tissue
