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Stephan Günther, Heinz Feldmann, Thomas W. Geisbert, Lisa E. Hensley, Pierre E. Rollin, Stuart T. Nichol, Ute Ströher, Harvey Artsob, Clarence J. Peters, Thomas G. Ksiazek, Stephan Becker, Jan ter Meulen, Stephan Ölschläger, Jonas Schmidt-Chanasit, Hinrich Sudeck, Gerd D. Burchard, Stefan Schmiedel, Management of Accidental Exposure to Ebola Virus in the Biosafety Level 4 Laboratory, Hamburg, Germany, The Journal of Infectious Diseases, Volume 204, Issue suppl_3, November 2011, Pages S785–S790, https://doi.org/10.1093/infdis/jir298
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Abstract
A needlestick injury occurred during an animal experiment in the biosafety level 4 laboratory in Hamburg, Germany, in March 2009. The syringe contained Zaire ebolavirus (ZEBOV) mixed with Freund’s adjuvant. Neither an approved treatment nor a postexposure prophylaxis (PEP) exists for Ebola hemorrhagic fever. Following a risk–benefit assessment, it was recommended the exposed person take an experimental vaccine that had shown PEP efficacy in ZEBOV-infected nonhuman primates (NHPs) [12]. The vaccine, which had not been used previously in humans, was a live-attenuated recombinant vesicular stomatitis virus (recVSV) expressing the glycoprotein of ZEBOV. A single dose of 5 × 107 plaque-forming units was injected 48 hours after the accident. The vaccinee developed fever 12 hours later and recVSV viremia was detectable by polymerase chain reaction (PCR) for 2 days. Otherwise, the person remained healthy, and ZEBOV RNA, except for the glycoprotein gene expressed in the vaccine, was never detected in serum and peripheral blood mononuclear cells during the 3-week observation period.
- polymerase chain reaction
- ebola virus
- fever
- glycoproteins
- accidents
- democratic republic of the congo
- freund's adjuvant
- genes
- germany
- ebola virus disease
- needlestick injuries
- primates
- vaccines
- vesicular stomatitis indiana virus
- vesiculovirus
- viremia
- syringes
- rna
- post-exposure prophylaxis
- peripheral blood mononuclear cell
- single-dose regimen
- laboratory biosafety level
- attenuation
- benefit-risk assessment