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Nico Marr, Alexey Novikov, Adeline M. Hajjar, Martine Caroff, Rachel C. Fernandez, Variability in the Lipooligosaccharide Structure and Endotoxicity among Bordetella pertussis Strains, The Journal of Infectious Diseases, Volume 202, Issue 12, 15 December 2010, Pages 1897–1906, https://doi.org/10.1086/657409
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Abstract
Bordetella endotoxins show remarkable structural variability both among each other and in comparison to other gram-negative bacteria. Here we demonstrate that, in contrast to the common Bordetella pertussis laboratory strain and Tohama I derivative BP338, lipooligosaccharide from mouse challenge strain 18-323 is a poor inducer of inflammatory cytokines in human and murine macrophages, is greatly impaired in Tolllike receptor 4-mediated activation of nuclear factor-κB in transfected HEK-293 cells, and functions as a Tolllike receptor 4 antagonist. Comparison of lipid A and lipooligosaccharide structures of B. pertussis strains BP338 and 18-323 revealed that 18-323 (1) lacks the ability to modify its lipid A phosphate groups with glucosamine, (2) is distinct in its acylation at the C3′ position of the lipid A diglucosamine backbone, and (3) expresses molecular lipooligosaccharide species that lack a terminal heptose. Our findings have important implications for interpreting previous studies of host defenses to B. pertussis infection in mice and in vitro.
