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Minsheng Xia, Robert J. Suchland, Roger E. Bumgarner, Tao Peng, Daniel D. Rockey, Walter E. Stamm, Chlamydia trachomatis Variant with Nonfusing Inclusions: Growth Dynamic and Host-Cell Transcriptional Response, The Journal of Infectious Diseases, Volume 192, Issue 7, 1 October 2005, Pages 1229–1236, https://doi.org/10.1086/444394
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Abstract
We compared growth rate and host-cell transcriptional responses of a Chlamydia trachomatis variant strain and a prototype strain. Growth dynamics were estimated by 16S rRNA level and by inclusion-forming units (IFUs) at different times after infection in HeLa cells. When inoculated at the same multiplicity of infection and observed 24–48 h after infection, the variant 16S rRNA transcriptional level was 3%–4% that of the prototype, and the IFUs of the variant strain were 0.1%–1% those of the prototype. Specific host-cell transcriptional responses to the variant were identified in a global-expression microarray in which variant strain–infected cells were compared with mock-infected and prototype strain–infected cells. In variant strain–infected cells, 47% (16/34) of specifically induced host genes were related to immunity and 32% (8/25) of specifically suppressed genes were related to lipid metabolism. The variant strain grew significantly more slowly and induced a modified host-cell transcriptional response, compared with the prototype strain
