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Takeshi Azuma, Shiho Yamazaki, Akiyo Yamakawa, Masahiro Ohtani, Atsushi Muramatsu, Hiroyuki Suto, Yoshiyuki Ito, Manabu Dojo, Yukinao Yamazaki, Masaru Kuriyama, Yoshihide Keida, Hideaki Higashi, Masanori Hatakeyama, Association between Diversity in the Src Homology 2 Domain-Containing Tyrosine Phosphatase Binding Site of Helicobacter pylori CagA Protein and Gastric Atrophy and Cancer, The Journal of Infectious Diseases, Volume 189, Issue 5, 1 March 2004, Pages 820–827, https://doi.org/10.1086/381782
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Abstract
We investigated the relationship between the diversity of Helicobacter pylori CagA protein and clinical outcome. The cagA gene was sequenced in 115 clinical isolates. The binding affinity of CagA to Src homology 2 domain-containing tyrosine phosphatase (SHP-2) was examined by in vitro infection. Two major CagA subtypes were observed—the East Asian and the Western type. The grades of inflammation, activity of gastritis, and atrophy were significantly higher in patients with gastritis infected with the East Asian CagA-positive strain than in patients with gastritis infected with cagA-negative or Western CagA-positive strains. All strains isolated from patients with gastric cancer were East Asian CagA positive. East Asian CagA exhibited stronger SHP-2-binding activity than did Western CagA. These findings suggest that infection with East Asian CagA-positive H. pylori is associated with atrophic gastritis and gastric cancer and that persistent active inflammation induced by the East Asian CagA-positive strain may play a role in the pathogenesis of disease.