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Craig Murdoch, Robert C. Read, Qibo Zhang, Adam Finn, Choline-Binding Protein A of Streptococcus pneumoniae Elicits Chemokine Production and Expression of Intercellular Adhesion Molecule 1 (CD54) by Human Alveolar Epithelial Cells, The Journal of Infectious Diseases, Volume 186, Issue 9, 1 November 2002, Pages 1253–1260, https://doi.org/10.1086/344527
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Abstract
Recruitment of neutrophils into alveolar air spaces is an early event in the pathogenesis of pneumonia due to Streptococcus pneumoniae. This results from chemokines released by activated endothelial and epithelial cells and alveolar macrophages. Culture supernatants of 6 wild-type strains of S. pneumoniae shown to contain choline-binding protein A (CbpA; clades A and B), induced release of chemokine CXCL8 from the human alveolar epithelial cell line A549, whereas a CbpA deletion mutant elicited significantly reduced CXCL8 release, compared with that of its isogenic parent (P<.01). Recombinant CbpA up-regulated expression of messenger RNA of CXCL8 and CCL2 but not of XCL1, CXCL10, CCL1, CCL3, CCL4, or CCL5 in A549 cells and induced increased secretion of CXCL8, CCL2, CXCL1, and CXCL5 in a dose- and time-dependent manner. CbpA also increased the expression of intercellular adhesion molecule 1 (CD54) by A549 cells. Thus, CbpA of S. pneumoniae induces the transcription and release of proinflammatory molecules by human alveolar epithelial cells
- chemokines
- bodily secretions
- cell lines
- choline
- endothelium
- intercellular adhesion molecule 1
- interleukin-8
- macrophages, alveolar
- monocyte chemoattractant protein-1
- neutrophils
- pneumonia, pneumococcal
- rantes
- rna, messenger
- staphylococcal protein a
- streptococcus pneumoniae
- carbon tetrachloride
- epithelial cells
- alveolar air
- molecule
- chemokine (c-x-c motif) ligand 1
- alveolar epithelium
- a549 cells