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Sandra M. Arend, Krista E. van Meijgaarden, Kirsten de Boer, Elisabeth Cerdá de Palou, Dick van Soolingen, Tom H. M. Ottenhoff, Jaap T. van Dissel, Tuberculin Skin Testing and In Vitro T Cell Responses to ESAT-6 and Culture Filtrate Protein 10 after Infection with Mycobacterium marinum or M. kansasii, The Journal of Infectious Diseases, Volume 186, Issue 12, 15 December 2002, Pages 1797–1807, https://doi.org/10.1086/345760
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Abstract
T cell responses to ESAT-6 and culture filtrate protein 10 (CFP-10), antigens expressed by Mycobacterium tuberculosis but not by M. bovis bacille Calmette-Guérin (BCG), were found to discriminate reliably between infection with M. tuberculosis and BCG vaccination. Because the esat-6 and cfp-10 genes occur in M. kansasii and M. marinum T cell responses to ESAT-6 and CFP-10 were investigated in patients infected with M. kansasii or M. marinum persons intensively exposed to environmental mycobacteria, and unexposed control subjects. Tuberculin skin tests were performed, and peripheral blood mononuclear cells were cocultured with ESAT-6, CFP-10, peptide mixtures of ESAT-6 and CFP-10, and control antigens. When enzyme-linked immunosorbent assay (ELISA) and enzyme-linked immunospot assay (ELISPOT) were used to measure interferon-γ production, most M. kansasii– or M. marinum–infected patients and several persons exposed to environmental mycobacteria were found to respond to ESAT-6 and/or CFP-10. ELISA and ELISPOT yielded comparable results, as did whole antigen and peptides (P<.0001). These results may be relevant for the development of novel assays for diagnosis of tuberculosis
- enzyme-linked immunosorbent assay
- bacille calmette-guerin
- bacille calmette-guerin vaccine
- antigens
- genes
- interferons
- mycobacterium
- mycobacterium marinum
- mycobacterium tuberculosis
- peptides
- hypersensitivity skin testing
- t-lymphocytes
- tuberculin
- tuberculin test
- tuberculosis
- infections
- diagnosis
- enzymes
- human leukocyte interferon
- enzyme linked immunospot assay
- peripheral blood mononuclear cell