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Anne Charlotte Grüner, Karima Brahimi, Franck Letourneur, Laurent Rénia, Wijnand Eling, Georges Snounou, Pierre Druilhe, Expression of the Erythrocyte-Binding Antigen 175 in Sporozoites and in Liver Stages of Plasmodium falciparum, The Journal of Infectious Diseases, Volume 184, Issue 7, 1 October 2001, Pages 892–897, https://doi.org/10.1086/323394
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Abstract
Screening of a Plasmodium falciparum genomic expression library for antigens expressed at the pre-erythrocytic stages resulted in the isolation of a recombinant phage (DG249) whose insert corresponded to regions II and III of a 175-kDa erythrocyte-binding antigen (EBA-175). EBA-175 is a parasite ligand implicated in red blood cell invasion. Reverse-transcriptase polymerase chain reaction, indirect immunofluorescent antibody test, and Western blot analysis confirmed that EBA-175 is expressed not only in blood-stage parasites but also in infected hepatocytes and on the sporozoite surface. The presence of EBA-175 on pre-erythrocytic parasites enhances the vaccine potential of this antigen by adding another target to the immune responses elicited by immunization