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Paul D. Fey, Joseph S. Ulphani, Friedrich Götz, Christine Heilmann, Dietrich Mack, Mark E. Rupp, Characterization of the Relationship between Polysaccharide Intercellular Adhesin and Hemagglutination in Staphylococcus epidermidis, The Journal of Infectious Diseases, Volume 179, Issue 6, June 1999, Pages 1561–1564, https://doi.org/10.1086/314762
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Abstract
To determine whether a relationship exists between biofilm formation and hemagglutination in Staphylococcus epidermidis, 20 skin isolates and 19 prosthetic valve endocarditis isolates were characterized for biofilm formation, hemagglutination, and the presence of a 357-bp polymerase chain reaction product within icaA. A strong association existed between biofilm formation, which has been linked to strains that produce polysaccharide intercellular adhesin (PIA), and hemagglutination. Strains that produced biofilm were significantly (P < .001 more likely to mediate hemagglutination (16 biofilm-positive/hemagglutination-positive strains and 19 biofilm-negative/hemagglutination-negative strains) within the 39 clinical strains tested. In addition, Staphylococcus carnosus TM300, a biofilm-negative, hemagglutination-negative strain, carrying the ica operon-containing plasmid pCN27, produced significant biofilm on glass and mediated hemagglutination (⩾1/128). It was concluded that production of PIA and hemagglutination are strongly associated and that PIA, at least in part, mediates hemagglutination in S. epidermidis.