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John G. Mastronarde, Marty M. Monick, Naofumi Mukaida, Kouji Matsushima, Gary W. Hunninghake, Activator Protein-1 Is the Preferred Transcription Factor for Cooperative Interaction with Nuclear Factor-κB in Respiratory Syncytial Virus-Induced Interleukin-8 Gene Expression in Airway Epithelium, The Journal of Infectious Diseases, Volume 177, Issue 5, May 1998, Pages 1275–1281, https://doi.org/10.1086/515279
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Abstract
The role of “oxidant-sensitive” transcription factors activator protein (AP)-1, nuclear factor (NF)-κB, and NF-IL6 in respiratory syncytial virus (RSV)-induced interleukin (IL)-8 gene expression in A549 epithelial cells was evaluated. RSV infection resulted in increased binding of each of these transcription factors. Transfection of A549 cells with plasmids containing serial truncations of the 5′-flanking region of the IL-8 gene revealed a positive cooperative effect of the binding sites for AP-1 and NF-κB. Mutation of either region markedly diminished responsiveness of the promoter to RSV. Mutation of the NF-IL6 site had minimal effect in the presence of intact binding sites for NF-κB and AP-1. The antioxidants NAC (N-acetylcysteine), DMSO, and DMPO (5,5-dimethyl-1-pyrroline N-oxide) did not inhibit RSV-induced binding of NF-κB; however, binding of AP-1 and NF-IL6 was inhibited. These observations suggest that AP-1 may be the preferred transcription factor (over NF-IL6) for cooperative interaction with NF-κB in RSV-induced IL-8 production.
- antioxidants
- gene expression
- mutation
- plasmids
- acetylcysteine
- epithelium
- binding sites
- ccaat-enhancer-binding protein-beta
- dimethyl sulfoxide
- genes
- interleukin-8
- interleukins
- oxidants
- oxides
- respiratory syncytial virus infections
- respiratory syncytial viruses
- transcription factor ap-1
- transfection
- transcription factor
- viruses
- transcriptional activation
- epithelial cells
- airway device
- airway structure
