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Robert S. Lowe, Darron R. Brown, Janine T. Bryan, James C. Cook, Hugh A. George, Kathryn J. Hofmann, William M. Hurni, Joseph G. Joyce, E. Dale Lehman, Henry Z. Markus, Michael P. Neeper, Loren D. Schultz, Alan R. Shaw, Kathrin U. Jansen, Human Papillomavirus Type 11 (HPV-11) Neutralizing Antibodies in the Serum and Genital Mucosal Secretions of African Green Monkeys Immunized with HPV-11 Virus-like Particles Expressed in Yeast, The Journal of Infectious Diseases, Volume 176, Issue 5, November 1997, Pages 1141–1145, https://doi.org/10.1086/514105
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Abstract
It has been shown previously that immunization of animals with recombinant virus-like particles (VLPs) consisting of the viral capsid proteins L1 or L1 plus L2 protected animals against experimental viral challenge. However, none of these experimental models addresses the issue of whether systemic immunization with VLPs elicits a neutralizing antibody response in the genital mucosa. Such a response may be necessary to protect the uterine cervix against infection with genital human papillomavirus (HPV) types. African green monkeys systemically immunized with HPV-11 VLPs expressed in Saccharomyces cerevisiae and formulated on aluminum adjuvant elicited high-titered HPV-11 VLP-specific serum antibody responses. Sera from these immunized monkeys neutralized HPV-11 in the athymic mouse xenograft system. Significant levels of HPV-11-neutralizing antibodies also were observed in cervicovaginal secretions. These findings suggest that protection against HPV infection of the uterine cervix may be possible through systemic immunization with HPV VLPs.
- human papillomavirus
- immunologic adjuvants
- pharmaceutical adjuvants
- antibody formation
- bodily secretions
- capsid
- cercopithecidae
- immunization
- mice, nude
- transplantation, heterologous
- yeasts
- infections
- aluminum
- antibodies
- cervix uteri
- mucous membrane
- viruses
- genital system
- serum
- neutralizing antibodies
- human papillomavirus 11