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Karl V. Sitz, Lawrence D. Loomis Price, Silvia Ratto Kim, Julie R. Kenner, Purnima Sau, Kenneth H. Eckels, Robert R. Redfield, Deborah L. Birx, Delayed-Type Hypersensitivity Skin Testing using Third Variable Loop Peptides Identifies T Lymphocyte Epitopes in Human Immunodeficiency Virus-Infected Persons, The Journal of Infectious Diseases, Volume 176, Issue 4, October 1997, Pages 1085–1089, https://doi.org/10.1086/516517
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Abstract
Cellular immune responses to human immunodeficiency virus type 1 (HIV-1) infection, particularly in vivo responses, have been difficult to study in large patient cohorts because of technical impediments. By use of small peptide fragments of the HIV-1 gp120 third variable loop, the CD4 T lymphocyte epitopes of 2 HIV-infected persons were mapped using a cutaneous delayed-type hypersensitivity (DTH) assay. The in vivo DTHresponses correlated with epitopes previously identified in vitro using CD4 T lymphocyte lines. The ability to determine CD4 T lymphocyte epitopes in large cohorts of patients using this simple in vivo technique would provide important diagnostic and prognostic data regarding effective immunoregulation of HIV-1. This technique should have broad applicability in HIV vaccine development and in the investigation of other immune-mediated human diseases.
- immune response
- aids vaccines
- epitopes
- t-lymphocyte epitopes
- hiv envelope protein gp120
- hiv-1
- delayed hypersensitivity
- immunologic deficiency syndromes
- peptide fragments
- peptides
- hypersensitivity skin testing
- t-lymphocytes
- infections
- diagnosis
- patient prognosis
- viruses
- immunomodulation
- hiv infections