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Patrizia Bagnarelli, Marco Candela, Anna Valenza, Aldo Manzin, Laura Solforosi, Francesca Mazzola, Luca Butini, Maria Montroni, Armando Gabrielli, Pietro E. Varaldo, Massimo Clementi, Dynamic Features of Human Immunodeficiency Virus Type 1 (HIV-1) Viremia: Kinetics of Cell-Free HIV-1 RNA after Therapeutic Plasma Exchange, The Journal of Infectious Diseases, Volume 176, Issue 3, September 1997, Pages 801–804, https://doi.org/10.1086/517306
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Abstract
To gain insight into the variables that influence the dynamics of human immunodeficiency virus type 1 (HIV-1) viremia levels, HIV-1 RNA molecules were quantified in plasma from an infected patient undergoing therapeutic plasma exchange (TPEx). After each TPEx procedure (2000 mL of fluid exchanged per session), HIV-1 genome molecule levels dropped to 58%–63% of the basal level but rapidly reverted to pre-TPEx values (doubling time = 3.50–4.04 h). Of interest, mobilization of extravascular cell-free virions (on average, 5.15 × 104 viral genome molecules/h) had already occurred during TPEx. After three daily TPEx procedures, HIV-1 viremia rebounded to basal values, while HIV-1 proviruses and viral transcripts in peripheral blood lymphocytes constantly tested at stable levels. Overall, this study extends previous analyses of the rate of HIV-1 turnover, using an alternative approach to the use of antiretroviral drugs, and it provides, albeit indirectly, insights into the amount and dynamic features of extravascular cell-free virus.