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Catherine Diamond, Meei-Li Huang, Dean H. Kedes, Carl Speck, George W. Rankin, Don Ganem, Robert W. Coombs, Timothy M. Rose, John N. Krieger, Lawrence Corey, Absence of Detectable Human Herpesvirus 8 in the Semen of Human Immunodeficiency Virus-Infected Men without Kaposi's Sarcoma, The Journal of Infectious Diseases, Volume 176, Issue 3, September 1997, Pages 775–777, https://doi.org/10.1086/517299
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Abstract
The prevalence of human herpesvirus 8 (HHV-8)/Kaposi's sarcoma (KS)-associated herpesvirus was investigated in the semen of 99 human immunodeficiency virus (HIV)-infected men (median CD4 cell count, 357/mm3) by use of a polymerase chain reaction (PCR) assay capable of detecting <10 copies of HHV-8 DNA. Of the subjects, 95 (96%) self-identified as men who have sex with men (MSM), and 3 had a history of clinical KS. Seminal cell specimens were negative for HHV-8 in 98 subjects. None of the 26 without KS (27.1% of 96 tested) who were seropositive for HHV-8 by IFA for latency-associated nuclear antigens had HHV-8 detected in their semen. The only subject with any evidence for seminal HHV-8 DNA was seropositive for HHV-8 and had active KS. HHV-8 was detected in 10 (10.4%) of 96 peripheral blood mononuclear cell specimens. The prevalence of HHV-8 DNA by PCR in semen of HIV-infected MSM without KS is low.