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Pawel Goluszko, Vsevolod Popov, Rangaraj Selvarangan, Stella Nowicki, Tuan Pham, Bogdan J. Nowicki, Dr Fimbriae Operon of Uropathogenic Escherichia coli Mediate Microtubule-Dependent Invasion to the HeLa Epithelial Cell Line, The Journal of Infectious Diseases, Volume 176, Issue 1, July 1997, Pages 158–167, https://doi.org/10.1086/514018
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Abstract
Escherichia coli Dr adhesin and decay-accelerating factor (DAF) receptor-mediated interaction was proposed as the mechanism of ascending urinary tract infection (UTI) and chronic interstitial nephritis. This report provides novel evidence for Dr fimbriae operon-mediated invasive capacity of Dr+E. coli. Insertional mutants draE, draC, and draB, and adherent draD and UV-inactivated BN406 were unable to enter HeLa cells. Complementation of the dra mutation restored invasiveness. Internalization was inhibited by anti-Dr fimbriae IgG (100%), anti-SCR-3 domain of DAF (75%), and nocodazole (95%). Increased receptor-ligand density occurred at the site of internalization. Internalized Dr+E. coli did not significantly multiply in the HeLa cell line. Accordingly, the dra operon and DAF were required for microtubule-dependent internalization of E. coli to HeLa cells. The relatively low invasion and multiplication rates of Dr+ E. coli may hypothetically contribute to the postattachment steps of ascending UTI and chronic renal infection.