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Brenda M. Diethelm-Okita, Raghavanpillai Raju, David K. Okita, Bianca M. Contl-Fine, Epitope Repertoire of Human CD4+ T Cells on Tetanus Toxin: Identification of Immunodominant Sequence Segments, The Journal of Infectious Diseases, Volume 175, Issue 2, February 1997, Pages 382–391, https://doi.org/10.1093/infdis/175.2.382
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Abstract
Sequence regions of tetanus toxin-forming CD4+ cell epitopes in 8 HLA-disparate subjects were identified. Overlapping synthetic peptides corresponding to the complete tetanus toxin sequence were used to test, in a proliferation assay, unselected blood CD4+ cells or CD4+ cell lines propagated by stimulation with tetanus toxoid. The CD4+ cell lines recognized most peptides recognized by the blood CD4+ cells and they recognized additional peptides. Their responses were stronger than those of unselected blood CD4+ cells. Two peptides were recognized by all subjects: one largely overlapped a tetanus toxin sequence region previously identified as a “universal” T cell epitope. Thirteen other peptides elicited a CD4+ cell response in 6 or 7 of the 8 subjects, and another 10 elicited responses in 5 subjects.