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Douglas C. Powers, Janet E. McElhaney, Oscar A. Florendo, Mary C. Manning, Craig M. Upshaw, David W. Bentley, Bethanie E. Wilkinson, Humoral and Cellular Immune Responses following Vaccination with Purified Recombinant Hemagglutinin from Influenza A (H3N2) Virus, The Journal of Infectious Diseases, Volume 175, Issue 2, February 1997, Pages 342–351, https://doi.org/10.1093/infdis/175.2.342
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Abstract
Adults were immunized with either baculovirus-expressed, purified recombinant hemagglutinin (rHA) from influenza A/Beijing/32/92 (H3N2) virus or saline placebo and evaluated for humoral and in vitro cellular immune responses. Compared with responses in placebo recipients, vaccinees had greater postvaccination H3Beijing/32 HA (H3)-specific lymphoproliferation and interleukin (IL)2, IL-l0, and interferon-γ (1FN-γ) production. Mean increases in the production of IL-l0 (⩾20-fold) and IL-2 (l0-fold) were relatively greater than that of IFN-γ (4-fold) or IL-4 (no change). Serum H3 antibodies were induced in 80% of rHA recipients, and the rise in antibody titer was significantly correlated with changes in IL-2, IL-l0, and IFN-γ concentrations. Vaccination with rHA only minimally enhanced anti-influenza virus cytotoxic T lymphocyte activity. These data demonstrate that rHA immunization of adults elicits a significant recall response by memory Band T lymphocytes and suggest that the cytokine response to vaccination has a T helper cell type 0-like profile.
- cytokine
- rheumatoid arthritis
- influenza
- aldesleukin
- immune response
- adult
- baculoviruses
- hemagglutinins
- immunization
- interferons
- interleukin-4
- interleukins
- mental recall
- t-lymphocytes
- t-lymphocytes, cytotoxic
- helper-inducer t-lymphocytes
- vaccination
- antibodies
- influenzavirus a
- memory
- viruses
- human leukocyte interferon
- normal saline
- saline solutions
- antibody titer
- beijing