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Reeta T. Mehta, Sourav Poddar, Mamta Kalidas, Ricardo Gomez-Flores, Kim Dulski, Role of Macrophages in the Candidacidal Activity of Liposomal Amphotericin B, The Journal of Infectious Diseases, Volume 175, Issue 1, January 1997, Pages 214–217, https://doi.org/10.1093/infdis/175.1.214
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Abstract
The role of macrophage activation in the candidacidal activity of liposome-incorporated (L) amphotericin B was investigated. Macrophages treated with L-amphotericin B killed Candida albicans more effectively than did macrophages treated with free (F) amphotericin B. However, macrophages treated with neither F- nor L-amphotericin B killed amphotericin B-resistant Candida tropicalis. In vivo stimulation of macrophages by intraperitoneal administration of thioglycollate, Freund's complete adjuvant, or heat-killed C. albicans followed by in vitro treatment with for L-amphotericin B, did not enhance their candidacidal activity. Intravenous administration of F- or L-amphotericin B did not augment the candidacidal activity of macrophages sensitized in vivo; however, sensitized macrophages showed enhanced killing compared with resident unstimulated cells. These studies suggest that macrophage-mediated enhancement of C. albicans killing may be due to uptake, transport, and delivery of L-amphotericin B to infected sites rather than to macrophage activation.