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Zorka Mikloska, Alison M. Kesson, Mark E. T. Penfold, Anthony L. Cunningham, Herpes Simplex Virus Protein Targets for CD4 and CD8 Lymphocyte Cytotoxicity in Cultured Epidermal Keratinocytes Treated with Interferon-γ, The Journal of Infectious Diseases, Volume 173, Issue 1, January 1996, Pages 7–17, https://doi.org/10.1093/infdis/173.1.7
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Abstract
In early recurrent herpetic lesions, CD4 T lymphocytes are the predominant infiltrating cells, and keratinocytes expressing major histocompatibility complex (MHC) class II antigens, induced by interferon-γ (IFN-γ), are the major site of herpes simplex virus (HSV)replication. IFN-γ pretreatment of human keratinocytes in vitro reduced MHC class I antigen down-regulation by HSV-1 infection and induced expression of HLA-DR that was unaltered by subsequent HSV-1 infection. Incubation of these infected keratinocytes with phosphonoacetic acid (PAA) almost completely inhibited expression of four major HSV glycoproteins, although expression of early proteins was not affected. Weak CD8 T lymphocyte cytotoxicity against IFN-γ-stimulated, HLA-DR-expressing HSV-1-infected keratinocytes was consistently directed to the immediate early/early proteins (all 9 patients tested) but against late proteins to a lesser degree (4/9 patients). However, CD4 T lymphocyte cytotoxicity was much greater and directed predominantly against late HSV-1 glycoproteins (all 9 subjects tested) in these cells.
- simplexvirus
- epidermis
- glycoproteins
- human leukocyte antigens
- cd8-positive t-lymphocytes
- down-regulation
- human herpesvirus 1
- histocompatibility antigens class i
- histocompatibility antigens class ii
- hla-dr antigens
- interferons
- keratinocytes
- major histocompatibility complex
- phosphonoacetic acid
- t-lymphocytes
- infections
- human leukocyte interferon
- cytotoxicity
- herpetic eruption