-
Views
-
Cite
Cite
Matthew Pollack, Ana Maria Espinoza, Gretchen Guelde, Nancy L. Koles, Larry M. Wahl, Christopher A. Ohl, Lipopolysaccharide (LPS)-Specific Monoclonal Antibodies Regulate LPS Uptake and LPS-Induced Tumor Necrosis Factor-α Responses by Human Monocytes, The Journal of Infectious Diseases, Volume 172, Issue 3, September 1995, Pages 794–804, https://doi.org/10.1093/infdis/172.3.794
- Share Icon Share
Abstract
Lipopolysaccharide (LPS)-monocytelmacrophage interactions are central to the infected host's inflammatory response to gram-negative bacteria. Flow cytometry was used to analyze the regulation by LPS-specific monoclonal antibodies (MAbs) of fluorescein isothiocyanate-conjugated LPS uptake by human peripheral blood monocytes. The uptake of LPS was stimulated by fresh or heat-inactivated serum (NHS or ΔNHS) or by LPS-binding protein and inhibited by α-LPS or α-CD14 (LPS receptor) MAbs. The inhibition by α-LPS MAbs of CD14-mediated LPS uptake was offset in the presence of NHS by a simultaneous MAb-mediated increase in LPS uptake that was blocked by α-complement receptor 1. Monocyte tumor necrosis factor-α responses to LPS were augmented by NHS and ΔNHS and inhibited by α-LPS MAbs. Thus, α-LPS MAbs down-regulate the proinflammatory uptake of LPS by human monocytes via membrane-bound CD14 while promoting complement- mediated opsonic uptake through membrane-associated CR1.