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Alex L. Laursen, Jørgen Rungby, Paul L. Andersen, Decreased Activation of the Respiratory Burst in Neutrophils from AIDS Patients with Previous Pneumocystis carinii Pneumonia, The Journal of Infectious Diseases, Volume 172, Issue 2, August 1995, Pages 497–505, https://doi.org/10.1093/infdis/172.2.497
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Abstract
Neutrophils from human immunodeficiency virus (HIV)-negative blood donors, asymptomatic HIV-positive patients, AIDS patients with previous Pneumocystis carinii pneumonia (PCP), and AIDS patients without previous PCP were compared for their ability to activate the respiratory burst, measured as luminol-amplified chemiluminescence. P. carinii, Staphylococcus aureus, phorbol-12-myristate-13-acetate, and FMLP were used to stimulate the neutrophils. When stimulated with P. carinii, neutrophils from PCP patients had a significantly lower response than the other groups, whereas no difference was found when S aureus was used. A somewhat but not significantly lower response to P. carinii was also seen in non-PCP patients compared with HIV-negative donors. Priming of the neutrophils with recombinant granulocyte colony-stimulating factor (G-CSF) or recombinant human granulocyte-macrophage (GM)-CSF corrected this defect. A similar effect of these cytokines was seen on phagocytosis, whereas the chemiluminescence in unprimed cells did not correlate with phagocytosis.
- cytokine
- hiv
- acquired immunodeficiency syndrome
- staphylococcus aureus
- acetates
- blood donors
- chemiluminescence
- recombinant granulocyte colony stimulating factor
- granulocytes
- hiv seropositivity
- luminol
- macrophages
- neutrophils
- n-formylmethionine leucyl-phenylalanine
- phagocytosis
- phorbols
- pneumonia, pneumocystis carinii
- respiratory burst
- donors