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Larry J. Anderson, Cecilia Tsou, Colin Potter, Harry L. Keyserling, Thomas F. Smith, Godwin Ananaba, Charles R. M. Bangham, Cytokine Response To Respiratory Syncytial Virus Stimulation Of Human, The Journal of Infectious Diseases, Volume 170, Issue 5, November 1994, Pages 1201–1208, https://doi.org/10.1093/infdis/170.5.1201
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Abstract
A key impediment to developing respiratory syncytial virus (RSV) vaccines is a lack of understanding of enhanced disease that occurred in children who received a formalin-inactivated RSV (FI-RSV) vaccine. Studies in mice have suggested that the FI-RSV vaccine induces a TH2 and live RSV induces a THI memory T cell response. In this study, the cytokine mRNA response of peripheral blood mononuclear cells (PBMC) from adults and children with and without previous RSV infection was characterized using a semiquantitative polymerase chain reaction (PCR). PBMC from 22 subjects previously infected with RSV usually had RSV-specific increases in THI cytokine-specific mRNA (interferon-ã [IFN-ã] mRNA, 20; interleukin [IL]-2 mRNA, 12; IL-5 mRNA, 6; and IL-4 mRNA, 0). PBMC from RSV antibody-negative children had no RSV-specific increases in IFN-ã IL-2, or IL-4 mRNA; 1 of 7 had an increase in IL-5 mRNA. These data indicate that naturally acquired RSV induces a TH1 memory T cell response.
- cytokine
- polymerase chain reaction
- aldesleukin
- adult
- child
- interferons
- interleukin-4
- interleukin-5
- interleukins
- respiratory syncytial virus infections
- respiratory syncytial viruses
- rna, messenger
- vaccines
- antibodies
- formaldehyde
- mice
- human leukocyte interferon
- peripheral blood mononuclear cell
- memory t cells