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C. S. Mclean, M. Erturk, R. Jennings, D. Ni Challanain, A. C. Minson, I. Duncan, M. E. G. Boursnell, S. C. Inglis, Protective Vaccination Against Primary And Recurrent Disease Caused By Herpes Simplex Virus (HSV) Type 2 Using A Genetically Disabled HSV-1, The Journal of Infectious Diseases, Volume 170, Issue 5, November 1994, Pages 1100–1109, https://doi.org/10.1093/infdis/170.5.1100
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Abstract
The vaccine potential of a mutant herpes simplex virus (HSV) type 1, with a deletion in the glycoprotein H (gH) gene, was evaluated. The virus requires a gH-expressing cell line for multi-cycle growth but can complete a single cycle of infection in noncomplementing cells. Such viruses, termed DISC (disabled infectious single cycle) viruses, should be safe, yet still able to stimulate humoral and cell-mediated responses against a broad range of virus antigens in vaccinated hosts. Prophylactic vaccination of guinea pigs with DISC HSV-1, by ear scarification or direct infection of the vaginal mucosa, afforded a high degree of protection against HSV-2-induced primary genital disease and reduced significantly the frequency of subsequent disease recurrence. There was also a trend toward reduced recurrence following therapeutic vaccination of animals already infected with HSV-2. DISC HSV vaccination, therefore, offers an effective route for control of HSV disease.