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Apurba K. Bhattacharjee, Steven M. Opal, John E. Palardy, Joseph J. Drabick, Hugh Collins, Robert Taylor, Amos Cotton, Alan S. Cross, Affinity-Purified Escherichia Coli J5 Lipopolysaccharide-Specific Igg Protects Neutropenic Rats Against Gram-Negative Bacterial Sepsis, The Journal of Infectious Diseases, Volume 170, Issue 3, September 1994, Pages 622–629, https://doi.org/10.1093/infdis/170.3.622
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Abstract
Antibodies were raised in rabbits by immunization with the heat-killed J5 mutant of Escherichia coli 0111 (Rc chemotype). Serum antibodies were separated into purified IgG and IgM by sequential affinity chromatography on protein G-Sepharose and anti-rabbit IgG-Sepharose columns. J5 lipopolysaccharide (LPS)-specific IgG was prepared by affinity chromatography of purified IgG on a J5 LPS-EAH Sepharose 4B affinity column. Purified IgM, IgG, and J5 LPS-specific IgG protected neutropenic rats against lethal challenge with Pseudomonas aeruginosa 12:4:4 (Fisher Devlin immunotype 6). Nine of 16 rats treated with the IgM fraction were protected (P < .001). Thirteen of 20 rats treated with the purified IgG and 6 of 8 treated with J5 LPS-specific IgG were protected compared with none of 25 treated with IgG made from the preimmune serum of the same rabbit (P < .001). These results demonstrate that purified J5 LPS-specific IgG protects against the lethal consequences of gram-negative bacteremia.
- pseudomonas aeruginosa
- sepsis
- neutropenia
- chromatography, affinity
- heat (physical force)
- immunization
- immunophenotyping
- lipopolysaccharides
- oryctolagus cuniculus
- sepharose
- immunoglobulin g
- immunoglobulin m
- antibodies
- rats
- gram-negative bacteremia
- escherichia coli
- affinity
- escherichia coli o111
- chemotype