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D. J. Conway, J. W. Bailey, J. F. Lindo, R. D. Robinson, D. A. P. Bundy, A. E. Bianco, Serum IgG Reactivity with 41-, 31-, and 28-kDa Larval Proteins of Strongyloides stercoralis in Individuals with Strongyloidiasis, The Journal of Infectious Diseases, Volume 168, Issue 3, September 1993, Pages 784–787, https://doi.org/10.1093/infdis/168.3.784
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Abstract
Proteins from a deoxycholate-soluble extract of Strongyloides stercoralis infective larvae were separated by SDS-PAGE, blotted onto nitrocellulose paper, and reacted with sera from individuals with confirmed S. stercoralis infections (n = 100), suspected S. stercoralis infections in whom no larvae could be detected (n = 27), and other nematode infections (40 with Wuchereria bancrofti, 20 with Onchocerca volvulus, 20 with Necator americanus, and 20 with mixed Ascaris lumbricoides and Trichuris trichiura infections). Immunodominant proteins of ∼41, 31, and 28 kDa were recognized by IgG in 91%,88%, and 90%, respectively, of sera from those with confirmed strongyloidiasis; in 100%, 100%, and 93% of sera from those with suspected strongyloidiasis; and in 9%, 12%, and 14% of sera from those infected with other nematodes. IgG reactivity to each of these proteins was a more specific means of immunodiagnosis than the currently used indirect ELISA; the methods were equally sensitive.
- enzyme-linked immunosorbent assay
- ascaris lumbricoides
- larva
- necator americanus
- nematoda
- nematode infections
- onchocerca volvulus
- strongyloides stercoralis
- strongyloidiasis
- trichuriasis
- wuchereria bancrofti
- immunoglobulin g
- infections
- deoxycholate
- serum
- sodium dodecyl sulfate-polyacrylamide gel electrophoresis