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Stuart T. Gunzburg, Barbara J. Chang, Simon J. Elliott, Valerie Burke, Michael Gracey, Diffuse and Enteroaggregative Patterns of Adherence of Enteric Escherichia coli Isolated from Aboriginal Children from the Kimberley Region of Western Australia, The Journal of Infectious Diseases, Volume 167, Issue 3, March 1993, Pages 755–758, https://doi.org/10.1093/infdis/167.3.755
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Abstract
Escherichia coli from 138 fecal samples from aboriginal children, in whom no other enteric pathogen was isolated (including enterovirulent E. coli), were examined for HEp-2 cell adhesion. Twenty-five (36.8%) of 68 children with diarrhea and 32 (45.7%) of 70 without diarrhea had diffusely adherent isolates, which were thus not associated with diarrhea (P > .25). However, after age stratification, children ⩾18 months showed a significant association of diffuselyadherent E. coli with diarrhea (P⩽.05). Enteroaggregative E. coli were isolated from 12 children with diarrhea (17.6%) and 15 without diarrhea (21.4%);thus, there was no association with diarrhea (P⩽.5). Sixteen children with diarrhea (23.5%) and 6 without diarrhea (8.6%) carried isolates that caused detachment of the HEp-2 cell monolayer from the glass coverslipwhen examined in the adhesion assay and were significantly associated with diarrhea (P⩽.05). These isolates, termed cell-detaching E. coli, were different from all recognized classes of enterovirulent E. coli.