-
Views
-
Cite
Cite
Joe P. Bryan, Maria H. Sjogren, Joseph L. Malone, Philip MacArthy, Tzu-Cheg Kao, Kenneth Wagner, Jaqueline Sheffield, Edith Smith, Peter L. Perine, Recombinant Immunoblot Assays for Hepatitis C in Human Immunodeficiency Virus Type 1-Infected US Navy Personnel, The Journal of Infectious Diseases, Volume 167, Issue 3, March 1993, Pages 715–719, https://doi.org/10.1093/infdis/167.3.715
- Share Icon Share
Abstract
The prevalence of hepatitis A, B, C, and D viruses was studied in 467 military personnel with human immunodeficiency virus type 1 (HIV-1) infection. Antibody to hepatitis C virus (antiHCV) by first-generation ELISA was found in 136 (29%). Of sera repeatedly reactive for antiHCV by first-generation ELISA, two-antigen recombinant immunoblot assay (RIBA) was positive in 41 (32%) and four-antigen RIBA was positive in 55 (41%). Four-antigen RIBA was positive in 33 (30%) ofthe 109 with an OD on ELISA of ⩽2.0 compared with 22 (81%) of the 27 with an OD >2.0 (P < .001). Anti-HCV detected by four-antigen RIBA was associated with increasing age, black or Hispanic race, and antibody to hepatitis B core antigen. When patients with hepatitis B surface antigen were excluded, elevated alanine aminotransferase was found in 5 (8%) of 63 with a negative RIBA and 13 (28%) of 47 with a positive RIBA (P = .006). While RIBA was negative in more than half of those with anti-HCV by ELISA, 55 (12%) of these HIV-1 infected personnel had anti-HCV detected by RIBA, which was associated with a strong reaction by ELISA, elevated liver enzymes, coinfection with hepatitis B, minority race, and older age.